OBJECTIVE Transforming growth factor β1 (TGF-β1) is a multifunc-tional cytokine that may play an important role in tumor development and progression. METHODS We evaluated gene expression patterns of TGF-β1 and its ...OBJECTIVE Transforming growth factor β1 (TGF-β1) is a multifunc-tional cytokine that may play an important role in tumor development and progression. METHODS We evaluated gene expression patterns of TGF-β1 and its receptors [transforming growth factor β type I receptor (TβR-Ⅰ) and transforming growth factor β type II receptor (TβR-Ⅱ)] in tumor tissue from patients with breast cancer or with benign breast diseases (BBD) and adja-cent normal tissue from the patients with breast cancer. Included in the study were 527 breast cancer patients and 213 BBD patients who participated in the Shanghai Breast Cancer Study. RESULTS The expression levels of the TGF-β1, TβR-Ⅰand TβR-Ⅱ genes in breast tissue were quantified using real-time PCR. TβR-Ⅱ expres-sion in cancer tissue was decreased by over 50% as compared to either adjacent normal tissue from the same patients or benign tumor tissue from BBD patients (p<0.001). TGF-β1 expression was lower by approximately 20% in cancer tissue compared to adjacent normal tissue (p=0.14) or to be-nign tumor tissue (p=0.002). Although TβR-Ⅰ expression was also reduced in cancer tissue compared to adjacent normal tissue, or benign tumor tissue, the magnitude of the reduction was less apparent than that for TβR-Ⅱ. Compared to patients with the lowest tertile value for TβR-Ⅱ, patients with median tertile value for TβR-Ⅱ had more favorable overall survival (HR 0.47, 95% CI 0.27-0.85) and disease-free survival (HR 0.65, 95% CI 0.39-1.06). No apparent associations, however, were observed between TGF-β1 or TβR-Ⅰ expression and overall or disease-free survival. CONCLUSION The results from this study support the hypothesis that a decreased level of TβR-Ⅱ gene expression, and thus reduced TGF-β1 sensitivity, is related to breast tumor progression.展开更多
Cu has been proved to possess various beneficial biological activities, while sandblasting and acid etching(SLA) is widely used to modify the commercial dental implant in order to improve osseointegration. Based on th...Cu has been proved to possess various beneficial biological activities, while sandblasting and acid etching(SLA) is widely used to modify the commercial dental implant in order to improve osseointegration. Based on the above, a novel antimicrobial dental implant material, Ti-Cu alloy, was treated with SLA, to combine chemical design(Cu addition) and topographical modification(SLA). In this work, the effects of SLA treated Ti-Cu alloys(Ti-Cu/SLA) on osteogenesis, angiogenesis and antibacterial properties were evaluated from both in vitro and in vivo tests, and Ti/SLA and Ti-Cu(without SLA) were served as control groups. Benefiting by the combined effects of chemical design(Cu addition) and micro-submicron hybrid structures(SLA),Ti-Cu/SLA had significantly improved inhibitory effects on oral anaerobic bacteria(P. gingivalis and S.mutans) and could induce upregulation of osteogenic-related and angiogenic-related genes expression in vitro. More importantly, in vivo studies also demonstrated that Ti-Cu/SLA implants had wonderful biological performance. In the osseointegration model, Ti-Cu/SLA implant promoted osseointegration via increasing peri-implant bone formation and presenting good bone-binding, compared to Ti/SLA and Ti-Cu implants. Additionally, in the peri-implantitis model, Ti-Cu/SLA effectively resisted the bone resorption resulted from bacterial infection and meanwhile promoted osseointegration. All these results suggest that the novel multiple functional Ti-Cu/SLA implant with rapid osseointegration and bone resorption inhibition abilities has the potential application in the future dental implantation.展开更多
基金a grant from the Science and Technology Commission of Shanghai Municipality (05JC14086)NIH grants RO1 CA64277 and RO1 CA90899 from the National Cancer Institute,USA.
文摘OBJECTIVE Transforming growth factor β1 (TGF-β1) is a multifunc-tional cytokine that may play an important role in tumor development and progression. METHODS We evaluated gene expression patterns of TGF-β1 and its receptors [transforming growth factor β type I receptor (TβR-Ⅰ) and transforming growth factor β type II receptor (TβR-Ⅱ)] in tumor tissue from patients with breast cancer or with benign breast diseases (BBD) and adja-cent normal tissue from the patients with breast cancer. Included in the study were 527 breast cancer patients and 213 BBD patients who participated in the Shanghai Breast Cancer Study. RESULTS The expression levels of the TGF-β1, TβR-Ⅰand TβR-Ⅱ genes in breast tissue were quantified using real-time PCR. TβR-Ⅱ expres-sion in cancer tissue was decreased by over 50% as compared to either adjacent normal tissue from the same patients or benign tumor tissue from BBD patients (p<0.001). TGF-β1 expression was lower by approximately 20% in cancer tissue compared to adjacent normal tissue (p=0.14) or to be-nign tumor tissue (p=0.002). Although TβR-Ⅰ expression was also reduced in cancer tissue compared to adjacent normal tissue, or benign tumor tissue, the magnitude of the reduction was less apparent than that for TβR-Ⅱ. Compared to patients with the lowest tertile value for TβR-Ⅱ, patients with median tertile value for TβR-Ⅱ had more favorable overall survival (HR 0.47, 95% CI 0.27-0.85) and disease-free survival (HR 0.65, 95% CI 0.39-1.06). No apparent associations, however, were observed between TGF-β1 or TβR-Ⅰ expression and overall or disease-free survival. CONCLUSION The results from this study support the hypothesis that a decreased level of TβR-Ⅱ gene expression, and thus reduced TGF-β1 sensitivity, is related to breast tumor progression.
基金financially supported by National Natural Science Foundation(Nos.51631009,51811530320 and 81572113)National Key Research and Development Program of China(Nos.2018YFC1106600 and 2016YFC1100600)+2 种基金Innovation Fund Project of Institute of Metal Research,Chinese Academy of Sciences(No.2017-ZD01)Key Projects for Foreign Cooperation of Bureau of International Cooperation Chinese Academy of Sciences(No.174321KYSB2018000)Shenzhen Science and Technology Research Funding(No.JCYJ20160608153641020)。
文摘Cu has been proved to possess various beneficial biological activities, while sandblasting and acid etching(SLA) is widely used to modify the commercial dental implant in order to improve osseointegration. Based on the above, a novel antimicrobial dental implant material, Ti-Cu alloy, was treated with SLA, to combine chemical design(Cu addition) and topographical modification(SLA). In this work, the effects of SLA treated Ti-Cu alloys(Ti-Cu/SLA) on osteogenesis, angiogenesis and antibacterial properties were evaluated from both in vitro and in vivo tests, and Ti/SLA and Ti-Cu(without SLA) were served as control groups. Benefiting by the combined effects of chemical design(Cu addition) and micro-submicron hybrid structures(SLA),Ti-Cu/SLA had significantly improved inhibitory effects on oral anaerobic bacteria(P. gingivalis and S.mutans) and could induce upregulation of osteogenic-related and angiogenic-related genes expression in vitro. More importantly, in vivo studies also demonstrated that Ti-Cu/SLA implants had wonderful biological performance. In the osseointegration model, Ti-Cu/SLA implant promoted osseointegration via increasing peri-implant bone formation and presenting good bone-binding, compared to Ti/SLA and Ti-Cu implants. Additionally, in the peri-implantitis model, Ti-Cu/SLA effectively resisted the bone resorption resulted from bacterial infection and meanwhile promoted osseointegration. All these results suggest that the novel multiple functional Ti-Cu/SLA implant with rapid osseointegration and bone resorption inhibition abilities has the potential application in the future dental implantation.
