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Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm 被引量:1
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作者 Kuisheng Liu Xiaocui Xu +16 位作者 Dandan Bai Yanhe Li Yalin Zhang Yanping Jia Mingyue Guo Xiaoxiao Han Yingdong Liu Yifan Sheng Xiaochen Kou Yanhong Zhao jiqing Yin Sheng Liu jiayu Chen Hong Wang Yixuan Wang Wenqiang Liu Shaorong Gao 《Protein & Cell》 SCIE CSCD 2023年第4期262-278,共17页
Self-organized blastoids from extended pluripotent stem(EPs)cells possess enormous potential for investigating postimplantation embryo development and related diseases.However,the limited ability of postimplantation d... Self-organized blastoids from extended pluripotent stem(EPs)cells possess enormous potential for investigating postimplantation embryo development and related diseases.However,the limited ability of postimplantation development of Eps-blastoids hinders its further application.In this study,single-cell transcriptomic analysis indicated that the“trophectoderm(TE)-like structure”of EPSblastoids was primarily composed of primitive endoderm(PrE)-related cells instead of TE-related cells.We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure.Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation.Furthermore,we demonstrated that blastocyst-like structures reconstituted by combining the EPs-derived bilineage embryo-like structure(BLEs)with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses.In summary,our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro. 展开更多
关键词 EPS cells blastoid primitive endoderm(PrE) trophectoderm(TE) Gata6
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