Objective: To explore the effect of Bismuth Subgallate/Borneol (Suile<sup>TM</sup> BSB) healing dressing combined with autologous platelet-rich gel (APG) in the treatment of diabetic foot ulcer (DFU). Meth...Objective: To explore the effect of Bismuth Subgallate/Borneol (Suile<sup>TM</sup> BSB) healing dressing combined with autologous platelet-rich gel (APG) in the treatment of diabetic foot ulcer (DFU). Methods: A total of 120 patients with DFU hospitalized in the Changsha Central Hospital from August 2020 to September 2021 were selected and randomly divided into an experimental group (BSB + APG, n = 60) and a control group (BSB, n = 60) according to random number table method. The total therapeutic effect, healing time, hospital stay, level indexes of various inflammatory factors before and after treatment and ulcer area were observed in the two groups. Results: The total effect of the control group was worse than that of the experimental group, and the data between the two groups were significant (P 0.05);after treatment, the levels of inflammatory factors including WBC, CRP, IL-6 and TNF-α in the control group were higher than those in the experimental group, and there was significant difference between the two groups (P 0.05);after 14 days of treatment, the ulcer area in the control group was larger than that in the experimental group, and the data between the two groups were significant (P Conclusion: BSB combined with APG can achieve better therapeutic effect, reduce the inflammatory reaction of patients, and promote wound healing in the treatment of patients with diabetic foot ulcer.展开更多
Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is...Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.展开更多
BACKGROUND The intestinal microcirculation functions in food absorption and metabolic substance exchanges.Accumulating evidence indicates that intestinal microcirculatory dysfunction is a significant source of multipl...BACKGROUND The intestinal microcirculation functions in food absorption and metabolic substance exchanges.Accumulating evidence indicates that intestinal microcirculatory dysfunction is a significant source of multiple gastrointestinal diseases.To date,there has not been a scientometric analysis of intestinal microcirculatory research.AIM To investigate the current status,development trends,and frontiers of intestinal microcirculatory research based on bibliometric analysis.METHODS VOSviewer and CiteSpace 6.1.R2 were used to identify the overall characteristics and knowledge map of intestinal microcirculatory research based on the core literature published from 2000 to 2021 in the Web of Science database.The characteristics of each article,country of origin,institution,journal,cocitations,and other information were analyzed and visualized.RESULTS There were 1364 publications enrolled in the bibliometric analysis,exhibiting an upward trend from 2000 to 2021 with increased participation worldwide.The United States and Dalhousie University took the lead among countries and institutions,respectively.Shock was the most prolific journal,and Nature Reviews Microbiology Clinical had the most citations.The topical hotspots and frontiers in intestinal microcirculatory research were centered on the pathological processes of functional impairment of intestinal microvessels,diverse intestinal illnesses,and clinical treatment.CONCLUSION Our study highlights insights into trends of the published research on the intestinal microcirculation and offers serviceable guidance to researchers by summarizing the prolific areas in intestinal disease research to date.展开更多
Objective Most patients with sepsis were diagnosed in the emergency department when admitted to the hospital. This study aimed to explore independent factors for hospital mortality in sepsis patients in the emergency ...Objective Most patients with sepsis were diagnosed in the emergency department when admitted to the hospital. This study aimed to explore independent factors for hospital mortality in sepsis patients in the emergency department.Methods It was a retrospective study. All patients with sepsis admitted to the emergency department of Changsha Central Hospital between 1 July 2020 and 1 July 2021 were included. Variables that showed significant differences between the survivor group and the non-survivor group were caculated in multivariate logistic regression analysis. Independent factors associated with in-hospital mortality in sepsis were identified.Results A total of 194 patients were included in this study. In-hospital mortality was 17.01%(n=33). Two independent risk factors including albumin(ALB) and urea nitrogen were identified by multivariate logistic regression analysis. The optimal cut-off values of ALB and urea nitrogen were 26.5g/L and 8.03mmol/L. While combined ALB and urea nitrogen, the area under the ROC curve(AUC) was 0.790, which was superior to the SOFA, qSOFA, and APACHEⅡscores.Conclusions Independent factors including ALB and urea nitrogen for in-hospital mortality in sepsis in the emergency department were identified. It could help physicians identify those patients with a higher risk of a poor prognosis early and easily, so intervention and management could be performed quickly.展开更多
Objective This study aimed to explore the association between uric acid(UA)and blood pressure(BP)in patients with diabetes receiving anti-diabetic therapy.Methods A cross-sectional study with 2878 individuals from the...Objective This study aimed to explore the association between uric acid(UA)and blood pressure(BP)in patients with diabetes receiving anti-diabetic therapy.Methods A cross-sectional study with 2878 individuals from the National Health and Nutrition Examination Survey(NHANES)was performed.Multiple linear regression analysis was applied to explore the relationship between UA and BP in the antihypertensive and anti-diabetic group(n=1966)and the anti-diabetic group(n=912).Results Between the group with antihypertensive and anti-diabetic therapy and the group with anti-diabetic therapy alone,no significant association was found with systolic blood pressure(SBP).However,the significantly negative association was present in diastolic blood pressure(DBP),and the size of the effect was(β,-0.35(95%CI:-0.68~-0.02))and(β,-0.60(95%CI:-1.13~-0.08)),respectively.The trend test was not significant between UA and SBP in two groups,but the trend test was significant between UA and DBP in the antihypertensive and anti-diabetic group(P=0.005).Conclusion In our study,we found that the association between UA and DBP in patients with diabetes receiving anti-diabetic therapy was negative.展开更多
Objective: As a rare cause of fever and lymphadenopathy (especially in the neck) in children, the purpose of this study is to explore the clinical characteristics of histiocytic necrotizing lymphadenitis (HNL) in chil...Objective: As a rare cause of fever and lymphadenopathy (especially in the neck) in children, the purpose of this study is to explore the clinical characteristics of histiocytic necrotizing lymphadenitis (HNL) in children and improve the diagnosis and treatment of this disease. Methods: We retrospectively collected the data of 6 pediatric patients with HNL diagnosed by lymph node biopsy from 2012 to 2021 in department of pediatric, the affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, including the clinical characteristics, laboratory examinations, imaging findings and histopathological examinations, treatment and follow-up of the patients and reviewed relevant literature. Results: All the patients were Han girls, with no kinship with each other, and four cases were from rural areas. The age of onset was from 7 to 14 years old, with durations ranged from 1 week to 3 months. All children had cervical lymphadenopathy with tenderness, five cases of fever, two cases of cough, one case of weight loss, four cases of leukopenia, one case of liver impairment, one case of combined bacterial infection of leukocytosis. All children underwent lymph node biopsy and were diagnosed with HNL, four children were treated with anti-infection before diagnosis, two children were treated with steroid therapy after diagnosis, and 1 case of weakened immune function was used immunoglobulin for modulating immunity. All children improved and discharged, and no recurrence was observed until October 2021. Conclusion: In our study, the children with swollen lymph nodes in the neck were accompanied by tenderness and fever as the main clinical manifestations, without specificity. Histopathology and immunohistochemical examination by removing the affected lymph nodes could confirm HNL. Most children had a good prognosis and long-term follow-up were required.展开更多
Background This study aimed to explore the associations of factors with in-hospital mortality in sepsis with MRSA infection based on the MIMIC-Ⅳdatabase.Methods All sepsis patients with MRSA infection were included.M...Background This study aimed to explore the associations of factors with in-hospital mortality in sepsis with MRSA infection based on the MIMIC-Ⅳdatabase.Methods All sepsis patients with MRSA infection were included.Multivariate regression analysis was applied to identify predictive factors.Models were applied to explore the relationship between the factors and in-hospital mortality after adjusting potential cofounders.Results A total of 157 patients were included including a survivor group(n=111)and a non-survivor group(n=46)were included.RDW was identified as an independent factor with in-hospital mortality.A smooth fitting curve for a positive linear relationship between RDW and in-hospital mortality was constructed.The area under the ROC curve(AUC)was 0.652 with a sensitivity of 0.565 and a specificity of 0.676.The optimal cut-off value of RDW was 16.55.Compared with SOFA and APACHEⅡscores,RDW had a better predictive performance.Conclusion RDW was associated with clinical outcomes in sepsis with MRSA infection.展开更多
Background Streptococcus pneumoniae,as a respiratory tract common pathogen,can cause invasive disease and sepsis.This study aimed to investigate the association of factors with clinical outcomes in sepsis with strepto...Background Streptococcus pneumoniae,as a respiratory tract common pathogen,can cause invasive disease and sepsis.This study aimed to investigate the association of factors with clinical outcomes in sepsis with streptococcus pneumoniae infection based on MIMIC-IV database.Methods The sepsis patients with streptococcus pneumoniae infection were included.Different variables between the survivor group and the non-survivor group were analyzed.Multivariable logistic regression was applied to identify the factors which were associated with clinical outcomes.Results A total of 80 sepsis patients with streptococcus pneumoniae infection were included.The in-hospital mortality was 23.75%(n=19).Significant differences were found in heart rate,white blood cell,RDW,MCV and hematocrit between the survivor group and the non-survivor group.The area under the ROC curve of hematocrit was 0.758 with a sensitivity of 73.7%and a specificity of 72.1%.The cut-off value of hematocrit was 30.8%.Conclusions Hematocrit level was identified to be negatively associated with in-hospital mortality in sepsis with streptococcus pneumoniae infection.展开更多
Background A model which can early and sensitively identify poor clinical outcome in short-term and long-term could be a useful tool to help physicians to assess the severity of the disease and early onset of therapeu...Background A model which can early and sensitively identify poor clinical outcome in short-term and long-term could be a useful tool to help physicians to assess the severity of the disease and early onset of therapeutic measures would be implemented in order to improve the prognosis of sepsis patients.This present study aimed to develop early predictive models for clinical outcomes based on a public database.Methods In the Medical Information Mart for Intensive Care-Ⅲ(MIMIC-Ⅲ)database,patients with severe sepsis or septic shock were included.Clinical variables were compared between survivor group and non-survivor group.Risk factors were identified by logistic regression model.Results A total of 2057 patients with severe sepsis or septic shock were finally enrolled.Mortality in 30-day and 180-day were 35.39%and 48.47%,respectively.Four independent factors including age,RDW,lactate and albumin for 30-day and 180-day mortality were identified in multivariate analysis.The accuracy of 30-day mortality model and 180-day mortality model were 0.702 and0.716,respectively.The area under the receiver operating characteristic curves(AUCs)of two models were 0.711 and 0.722,respectively.Conclusions In our study,apredictive model with four independent factors including age,RDW,lactate and albumin was performed by logistic regression,which could be applied for early identification in both 30-day and 180-day mortality in severe sepsis or septic shock.展开更多
文摘Objective: To explore the effect of Bismuth Subgallate/Borneol (Suile<sup>TM</sup> BSB) healing dressing combined with autologous platelet-rich gel (APG) in the treatment of diabetic foot ulcer (DFU). Methods: A total of 120 patients with DFU hospitalized in the Changsha Central Hospital from August 2020 to September 2021 were selected and randomly divided into an experimental group (BSB + APG, n = 60) and a control group (BSB, n = 60) according to random number table method. The total therapeutic effect, healing time, hospital stay, level indexes of various inflammatory factors before and after treatment and ulcer area were observed in the two groups. Results: The total effect of the control group was worse than that of the experimental group, and the data between the two groups were significant (P 0.05);after treatment, the levels of inflammatory factors including WBC, CRP, IL-6 and TNF-α in the control group were higher than those in the experimental group, and there was significant difference between the two groups (P 0.05);after 14 days of treatment, the ulcer area in the control group was larger than that in the experimental group, and the data between the two groups were significant (P Conclusion: BSB combined with APG can achieve better therapeutic effect, reduce the inflammatory reaction of patients, and promote wound healing in the treatment of patients with diabetic foot ulcer.
基金supported by the Science and Technology Innovation Program of Hunan Province(Grant Numbers:2021SK1014 and 2022WZ1027)the Colleges and Universities of Hunan Province(Grant Number:HNJG 20200440)+1 种基金the Scientific Research Fund of Hunan Provincial Education Department(Grant Number:21B0411)the Scientific Research Project of Changsha Central Hospital(Number:YNKY202201).
文摘Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.
基金Supported by the Beijing Municipal Natural Science Foundation,No.7212068the National Natural Science Foundation of China,No.81900747.
文摘BACKGROUND The intestinal microcirculation functions in food absorption and metabolic substance exchanges.Accumulating evidence indicates that intestinal microcirculatory dysfunction is a significant source of multiple gastrointestinal diseases.To date,there has not been a scientometric analysis of intestinal microcirculatory research.AIM To investigate the current status,development trends,and frontiers of intestinal microcirculatory research based on bibliometric analysis.METHODS VOSviewer and CiteSpace 6.1.R2 were used to identify the overall characteristics and knowledge map of intestinal microcirculatory research based on the core literature published from 2000 to 2021 in the Web of Science database.The characteristics of each article,country of origin,institution,journal,cocitations,and other information were analyzed and visualized.RESULTS There were 1364 publications enrolled in the bibliometric analysis,exhibiting an upward trend from 2000 to 2021 with increased participation worldwide.The United States and Dalhousie University took the lead among countries and institutions,respectively.Shock was the most prolific journal,and Nature Reviews Microbiology Clinical had the most citations.The topical hotspots and frontiers in intestinal microcirculatory research were centered on the pathological processes of functional impairment of intestinal microvessels,diverse intestinal illnesses,and clinical treatment.CONCLUSION Our study highlights insights into trends of the published research on the intestinal microcirculation and offers serviceable guidance to researchers by summarizing the prolific areas in intestinal disease research to date.
基金Changsha Natural Science Foundation(NO.kq2208445)Changsha Central Hospital (NO.YNKY202306)Changsha Science and Technology Bureau(NO.kzd21084, kzd22074, kzd22075)。
文摘Objective Most patients with sepsis were diagnosed in the emergency department when admitted to the hospital. This study aimed to explore independent factors for hospital mortality in sepsis patients in the emergency department.Methods It was a retrospective study. All patients with sepsis admitted to the emergency department of Changsha Central Hospital between 1 July 2020 and 1 July 2021 were included. Variables that showed significant differences between the survivor group and the non-survivor group were caculated in multivariate logistic regression analysis. Independent factors associated with in-hospital mortality in sepsis were identified.Results A total of 194 patients were included in this study. In-hospital mortality was 17.01%(n=33). Two independent risk factors including albumin(ALB) and urea nitrogen were identified by multivariate logistic regression analysis. The optimal cut-off values of ALB and urea nitrogen were 26.5g/L and 8.03mmol/L. While combined ALB and urea nitrogen, the area under the ROC curve(AUC) was 0.790, which was superior to the SOFA, qSOFA, and APACHEⅡscores.Conclusions Independent factors including ALB and urea nitrogen for in-hospital mortality in sepsis in the emergency department were identified. It could help physicians identify those patients with a higher risk of a poor prognosis early and easily, so intervention and management could be performed quickly.
文摘Objective This study aimed to explore the association between uric acid(UA)and blood pressure(BP)in patients with diabetes receiving anti-diabetic therapy.Methods A cross-sectional study with 2878 individuals from the National Health and Nutrition Examination Survey(NHANES)was performed.Multiple linear regression analysis was applied to explore the relationship between UA and BP in the antihypertensive and anti-diabetic group(n=1966)and the anti-diabetic group(n=912).Results Between the group with antihypertensive and anti-diabetic therapy and the group with anti-diabetic therapy alone,no significant association was found with systolic blood pressure(SBP).However,the significantly negative association was present in diastolic blood pressure(DBP),and the size of the effect was(β,-0.35(95%CI:-0.68~-0.02))and(β,-0.60(95%CI:-1.13~-0.08)),respectively.The trend test was not significant between UA and SBP in two groups,but the trend test was significant between UA and DBP in the antihypertensive and anti-diabetic group(P=0.005).Conclusion In our study,we found that the association between UA and DBP in patients with diabetes receiving anti-diabetic therapy was negative.
文摘Objective: As a rare cause of fever and lymphadenopathy (especially in the neck) in children, the purpose of this study is to explore the clinical characteristics of histiocytic necrotizing lymphadenitis (HNL) in children and improve the diagnosis and treatment of this disease. Methods: We retrospectively collected the data of 6 pediatric patients with HNL diagnosed by lymph node biopsy from 2012 to 2021 in department of pediatric, the affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, including the clinical characteristics, laboratory examinations, imaging findings and histopathological examinations, treatment and follow-up of the patients and reviewed relevant literature. Results: All the patients were Han girls, with no kinship with each other, and four cases were from rural areas. The age of onset was from 7 to 14 years old, with durations ranged from 1 week to 3 months. All children had cervical lymphadenopathy with tenderness, five cases of fever, two cases of cough, one case of weight loss, four cases of leukopenia, one case of liver impairment, one case of combined bacterial infection of leukocytosis. All children underwent lymph node biopsy and were diagnosed with HNL, four children were treated with anti-infection before diagnosis, two children were treated with steroid therapy after diagnosis, and 1 case of weakened immune function was used immunoglobulin for modulating immunity. All children improved and discharged, and no recurrence was observed until October 2021. Conclusion: In our study, the children with swollen lymph nodes in the neck were accompanied by tenderness and fever as the main clinical manifestations, without specificity. Histopathology and immunohistochemical examination by removing the affected lymph nodes could confirm HNL. Most children had a good prognosis and long-term follow-up were required.
文摘Background This study aimed to explore the associations of factors with in-hospital mortality in sepsis with MRSA infection based on the MIMIC-Ⅳdatabase.Methods All sepsis patients with MRSA infection were included.Multivariate regression analysis was applied to identify predictive factors.Models were applied to explore the relationship between the factors and in-hospital mortality after adjusting potential cofounders.Results A total of 157 patients were included including a survivor group(n=111)and a non-survivor group(n=46)were included.RDW was identified as an independent factor with in-hospital mortality.A smooth fitting curve for a positive linear relationship between RDW and in-hospital mortality was constructed.The area under the ROC curve(AUC)was 0.652 with a sensitivity of 0.565 and a specificity of 0.676.The optimal cut-off value of RDW was 16.55.Compared with SOFA and APACHEⅡscores,RDW had a better predictive performance.Conclusion RDW was associated with clinical outcomes in sepsis with MRSA infection.
文摘Background Streptococcus pneumoniae,as a respiratory tract common pathogen,can cause invasive disease and sepsis.This study aimed to investigate the association of factors with clinical outcomes in sepsis with streptococcus pneumoniae infection based on MIMIC-IV database.Methods The sepsis patients with streptococcus pneumoniae infection were included.Different variables between the survivor group and the non-survivor group were analyzed.Multivariable logistic regression was applied to identify the factors which were associated with clinical outcomes.Results A total of 80 sepsis patients with streptococcus pneumoniae infection were included.The in-hospital mortality was 23.75%(n=19).Significant differences were found in heart rate,white blood cell,RDW,MCV and hematocrit between the survivor group and the non-survivor group.The area under the ROC curve of hematocrit was 0.758 with a sensitivity of 73.7%and a specificity of 72.1%.The cut-off value of hematocrit was 30.8%.Conclusions Hematocrit level was identified to be negatively associated with in-hospital mortality in sepsis with streptococcus pneumoniae infection.
文摘Background A model which can early and sensitively identify poor clinical outcome in short-term and long-term could be a useful tool to help physicians to assess the severity of the disease and early onset of therapeutic measures would be implemented in order to improve the prognosis of sepsis patients.This present study aimed to develop early predictive models for clinical outcomes based on a public database.Methods In the Medical Information Mart for Intensive Care-Ⅲ(MIMIC-Ⅲ)database,patients with severe sepsis or septic shock were included.Clinical variables were compared between survivor group and non-survivor group.Risk factors were identified by logistic regression model.Results A total of 2057 patients with severe sepsis or septic shock were finally enrolled.Mortality in 30-day and 180-day were 35.39%and 48.47%,respectively.Four independent factors including age,RDW,lactate and albumin for 30-day and 180-day mortality were identified in multivariate analysis.The accuracy of 30-day mortality model and 180-day mortality model were 0.702 and0.716,respectively.The area under the receiver operating characteristic curves(AUCs)of two models were 0.711 and 0.722,respectively.Conclusions In our study,apredictive model with four independent factors including age,RDW,lactate and albumin was performed by logistic regression,which could be applied for early identification in both 30-day and 180-day mortality in severe sepsis or septic shock.
