The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which triggered the ongoing pandemic,was first discovered in China in late 2019.SARSCoV-2 is a respiratory virus responsible for coronavirus diseas...The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which triggered the ongoing pandemic,was first discovered in China in late 2019.SARSCoV-2 is a respiratory virus responsible for coronavirus disease 2019(COVID-19)that often manifests as a pneumonic syndrome.In the context of the pandemic,there are mixed views on the data provided by epidemiologists and the information collected by hospital clinicians about their patients.In addition,the literature reports a large proportion of patients free of pneumonia vs a small percentage of patients with severe pneumonia among confirmed COVID-19 cases.This raises the issue of the complexity of the work required to control or contain the pandemic.We believe that an integrative and pluralistic approach will help to put the analyses into perspective and reinforce collaboration and creativity in the fight against this major scourge.This paper proposes a comprehensive and integrative approach to COVID-19 research,prevention,control,and treatment to better address the pandemic.Thus,this literature review applies a pluralistic approach to fight the pandemic.展开更多
Human mesenchymal stem cells(hMSC)can be expanded in vitro and differentiated towards osteogenic,chondrogenic or adipogenic lineages,making them an attractive source for tissue engineering and regenerative medicine.Ch...Human mesenchymal stem cells(hMSC)can be expanded in vitro and differentiated towards osteogenic,chondrogenic or adipogenic lineages,making them an attractive source for tissue engineering and regenerative medicine.Chitinase-like-proteins(CLPs)belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling.The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC.Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC.YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation.A slight but non-significant increase in YKL-40 expression was observed with glucosamine,accompanied by a pH-dependent delay in mineralization.However,glucosamine induced higher expression of osteogenic marker genes.展开更多
文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which triggered the ongoing pandemic,was first discovered in China in late 2019.SARSCoV-2 is a respiratory virus responsible for coronavirus disease 2019(COVID-19)that often manifests as a pneumonic syndrome.In the context of the pandemic,there are mixed views on the data provided by epidemiologists and the information collected by hospital clinicians about their patients.In addition,the literature reports a large proportion of patients free of pneumonia vs a small percentage of patients with severe pneumonia among confirmed COVID-19 cases.This raises the issue of the complexity of the work required to control or contain the pandemic.We believe that an integrative and pluralistic approach will help to put the analyses into perspective and reinforce collaboration and creativity in the fight against this major scourge.This paper proposes a comprehensive and integrative approach to COVID-19 research,prevention,control,and treatment to better address the pandemic.Thus,this literature review applies a pluralistic approach to fight the pandemic.
文摘Human mesenchymal stem cells(hMSC)can be expanded in vitro and differentiated towards osteogenic,chondrogenic or adipogenic lineages,making them an attractive source for tissue engineering and regenerative medicine.Chitinase-like-proteins(CLPs)belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling.The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC.Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC.YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation.A slight but non-significant increase in YKL-40 expression was observed with glucosamine,accompanied by a pH-dependent delay in mineralization.However,glucosamine induced higher expression of osteogenic marker genes.
