Objective:The aim was to evaluate the role of whole body diffusion weighted imaging(WB-DWI) of magnetic resonance in the diagnosis and efficacy evaluation of malignant lymphoma.Methods:The clinical manifestation of wh...Objective:The aim was to evaluate the role of whole body diffusion weighted imaging(WB-DWI) of magnetic resonance in the diagnosis and efficacy evaluation of malignant lymphoma.Methods:The clinical manifestation of whole body diffusion weighted imaging of 47 patients with malignant lymphoma being pathologically proved were carried on by retrospective analysis and to compare with 10 healthy volunteers.There were 8 cases of Hodgkin's lymphoma(HD) patients,of which WB-DWI examination of 5 cases were carried out before and after treatment and 3 cases were done after treatment.There were 39 cases of non-Hodgkin's lymphoma(NHL) patients,of which WB-DWI examination in 19 cases were acted before treatment,11 cases were done before and after treatment and 9 cases done after treatment.In apparent diffusion coefficient(ADC) diagram the ADC values of lymph nodes in patients with malignant lymphoma and healthy volunteers were measured respectively,among 16 patients the ADC values of parts of lymph node being consistent with the initial inspection were determined again and compared the values before and after,and compared with healthy volunteers.Results:Whole body magnetic resonance diffusion weighted imaging(MR-DWI) showed more sensitive to lymphoma,and 372 lymph nodes greater than 1 cm were detected with MR-DWI.Before treatment,mean ADC value of 35 patients with malignant lymphoma was(0.86 ± 0.21) × 10-3 mm2/s,of which an average ADC values of 28 cases after treatment was(1.22 ± 0.31) × 10-3 mm2/s,before and after treatment difference of the average ADC values of patients was statistically significant(P < 0.05);the average ADC value of cervical lymph nodes of 10 healthy volunteers(1.29 ± 0.12) × 10-3 mm2/s and of 30 patients with malignant lymphoma before treatment was statistically significant(P < 0.05),and to compare with 28 patients after treatment difference of the ADC values was not statistically significant(P > 0.05).Conclusion:WB-DWI and ADC values being measured in the clinical diagnosis of malignant lymphoma,staging and evaluation of efficacy of monitoring is a fast and effective technology,with some clinical value.展开更多
Objective:To study the effect of hemodialysis (HD) combined with hemoperfusion (HP) on dialysis efficiency, lipid metabolism and atherosclerosis in patients with uremia.Methods:A total of 70 patients with uremia who w...Objective:To study the effect of hemodialysis (HD) combined with hemoperfusion (HP) on dialysis efficiency, lipid metabolism and atherosclerosis in patients with uremia.Methods:A total of 70 patients with uremia who were treated in our hospital between March 2013 and October 2015 were collected and divided into observation group and control group (n=35) according to double-blind randomized control method. Observation group of patients received hemodialysis combined with hemoperfusion, control group of patients received hemodialysis alone, and the treatment lasted for 6 months. After 6 months of intervention, automatic biochemical analyzer was used to detect renal function indexes and lipid metabolism indexes, and the two-dimensional ultrasound was used to quantitatively determine the carotid atherosclerosis parameters.Results:Before intervention, differences in renal function, lipid metabolism and atherosclerosis levels were not statistically significant between two groups of patients;after 6 months of intervention, renal function indexes blood urea nitrogen (BUN), serum creatinine (Scr),β2-microglobulin (β2-MG) and blood uric acid (BUA) levels of observation group were lower than those of control group, lipid metabolism indexes total cholesterol (TC), triglyceride (TG), apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) levels were lower than those of control group, and differences between groups were statistically significant;quantitative carotid ultrasound parameters gray-scale median (GSM) of observation group was higher than that of control group, enhanced intensity (EI) and enhanced density (ED) were lower than those of control group, and differences between groups were statistically significant.Conclusion:Hemodialysis combined with hemoperfusion can improve the dialysis efficiency, also reduce lipid metabolism disturbance and delay the formation of atherosclerosis in patients with uremia.展开更多
Objective:To study the correlation of Doppler ultrasound parameters with angiogenesis and cancer cell growth in endometrial cancer.Methods: Patients with endometrial cancer and fibroids who accepted surgical resection...Objective:To study the correlation of Doppler ultrasound parameters with angiogenesis and cancer cell growth in endometrial cancer.Methods: Patients with endometrial cancer and fibroids who accepted surgical resection in the Central Hospital of Enshi Autonomous Prefecture between March 2015 and June 2017 were selected as the research subjects and enrolled in the observation group and control group of the study respectively. The preoperative pelvic Doppler ultrasonography was done to determine the blood flow parameters resistance index (RI) and pulsatility index (PI) of the endometrial lesions;after surgical resection, the endometrial cancer lesion tissue was collected from the observation group and normal endometrial tissue was collected from the control group to determine the contents of pro-proliferation molecules, pro-invasion molecules and angiogenesis molecules.Results: PI and RI levels in endometrial cancer lesion of observation group were significantly lower than those of control group, and cIAP1, cIAP2, RLIP76, p-Pak4ser474, EFEMP2, CXCR4, CXCR7, Snail, Septin-9,β-arrestin2 and SOX2 protein levels in endometrial cancer lesion were significantly higher than those of control group;PI and RI levels in endometrial cancer lesion were negatively correlated with cIAP1, cIAP2, RLIP76, p-Pak4ser474, EFEMP2, CXCR4, CXCR7, Snail, Septin-9,β-arrestin2 and SOX2 protein levels.Conclusion: The Doppler ultrasound parameters of endometrial carcinoma lesion are closely related to the angiogenesis and cancer cell growth in the lesion.展开更多
Objective: To study the effect of trastuzumab combined with paclitaxel neoadjuvant chemotherapy on the cell proliferation and invasion in human epidermal growth factor receptor-2 (HER-2)-positive breast cancer lesions...Objective: To study the effect of trastuzumab combined with paclitaxel neoadjuvant chemotherapy on the cell proliferation and invasion in human epidermal growth factor receptor-2 (HER-2)-positive breast cancer lesions. Methods: Patients who were diagnosed with HER-2-positive breast cancer in the Central Hospital of Enshi Autonomous Prefecture Hubei Province between April 2015 and January 2017 were selected and randomly divided into two groups, the combined group received trastuzumab combined with paclitaxel chemotherapy, and the control group accepted paclitaxel chemotherapy. The surgically removed breast cancer lesions were collected to determine the expression of cell proliferation genes, cell invasion genes and angiogenesis molecules. Results: USP39, EphA2, NUAK1, Gab2, Raptor, ICAM-1, HIF-1α, VEGF, ANGPLT-2 and ANGPLT-4 mRNA expression in tumor lesion of combined group were significantly lower than those of control group while CCN5, ALEX1, ATG2B, ATG4D, E-cadherin and EBP50 mRNA expression were significantly higher than those of control group. Conclusion: Trastuzumab combined with paclitaxel neoadjuvant chemotherapy can inhibit the cell proliferation and invasion and decrease the angiogenesis in HER-2-positive breast cancer lesions.展开更多
Objective:To study the evaluation value of HMGA2, Tiam1 and Notch1 expression for cancer cell proliferation, invasion and epithelial-mesenchymal transition in non-small cell lung cancer tissue. Methods:Non-small cell ...Objective:To study the evaluation value of HMGA2, Tiam1 and Notch1 expression for cancer cell proliferation, invasion and epithelial-mesenchymal transition in non-small cell lung cancer tissue. Methods:Non-small cell lung cancer tissues and para-carcinoma tissues surgically removed in our hospital between May 2013 and December 2015 were collected, immunohistochemical staining method was used to determine HMGA2, Tiam1 and Notch1 protein expression, and fluorescence quantitative PCR was used to determine the mRNA expression of HMGA2, Tiam1, Notch1 as well as proliferation, invasion and epithelial-mesenchymal transition molecules. Results:HMGA2, Tiam1 and Notch1 mRNA expression and positive protein expression rate in lung cancer tissues were significantly higher than those in para-carcinoma tissues (P<0.05);E-cadherin mRNA expression in lung cancer tissues with positive HMGA2 expression was significantly lower than that in lung cancer tissues with negative HMGA2 expression (P<0.05) while N-cadherin and Vimentin mRNA expression were significantly higher than those in lung cancer tissues with negative HMGA2 expression (P<0.05);MMP2, MMP3, MMP8 and MMP9 mRNA expression in lung cancer tissues with positive Tiam1 expression were significantly higher than those in lung cancer tissues with negative Tiam1 expression (P<0.05);CyclinD1, Bcl-2 and Survivin mRNA expression in lung cancer tissues with positive Notch1 expression were significantly higher those in lung cancer tissues with negative Notch1 expression (P<0.05). Conclusions:Highly expressed HMGA2, Tiam1 and Notch1 in lung cancer tissues can regulate the epithelial-mesenchymal transition, invasion and proliferation of cancer cells respectively.展开更多
Objective: To measure the contrast-enhanced ultrasound parameters of endometrial benign and malignant lesions and investigate their correlation with malignant molecule expression. Methods: A total of 118 patients who ...Objective: To measure the contrast-enhanced ultrasound parameters of endometrial benign and malignant lesions and investigate their correlation with malignant molecule expression. Methods: A total of 118 patients who were examined and diagnosed with endometrial benign hyperplasia in the hospital between December 2012 and January 2017 were collected as endometrial hyperplasia group, and 67 patients with endometrial carcinoma were collected as endometrial cancer group. The differences in the levels of uterine contrast-enhanced ultrasound parameters as well as the expression of proliferation, apoptosis and invasion genes in endometrial lesion tissue were compared between the two groups. Pearson test was used to evaluate the correlation between uterine contrast-enhanced ultrasound parameters and malignant molecule expression in the lesions. Results: Contrast-enhanced ultrasound parameter TTP level of endometrial cancer group was lower than that of endometrial hyperplasia group whereas PI level was higher than that of endometrial hyperplasia group. Proliferation gene EFEMP2 mRNA expression in lesion tissue of endometrial cancer group was lower than that of endometrial hyperplasia group whereas RRM2 and DJ-1 mRNA expression were higher than those of endometrial hyperplasia group;apoptosis genes P53, c-myc and Livin mRNA expression were higher than those of endometrial hyperplasia group whereas Caspase-3 and Bid mRNA expression were lower than those of endometrial hyperplasia group;invasion gene DKK1 mRNA expression was lower than that of endometrial hyperplasia group whereas HMGB1, EZH2 and Snail mRNA expression were higher than those of endometrial hyperplasia group. Pearson test showed that the contrast-enhanced ultrasound parameter levels of endometrial cancer were directly correlated with the malignant molecule expression in the lesion tissues. Conclusion: contrast-enhanced ultrasound parameter levels can be used to identify endometrial benign and malignant lesions and are directly correlated with the severity of the lesion.展开更多
Objective: To study the regulating effect of Par-4 gene overexpression on the nephroblastoma sensitivity to cisplatin. Methods: Nephroblastoma SK-NEP-1 cells were cultured and divided into four groups, control group w...Objective: To study the regulating effect of Par-4 gene overexpression on the nephroblastoma sensitivity to cisplatin. Methods: Nephroblastoma SK-NEP-1 cells were cultured and divided into four groups, control group were treated with RMPI-1640 without serum or drugs, cisplatin group were treated with serum-free RMPI-1640 containing 5 μg/mL cisplatin, Par-4 group were transfected by Par-4 overexpression plasmids with serum-free RMPI-1640, and cisplatin+ Par-4 group were transfected by Par-4 overexpression plasmid with serum-free RMPI-1640 containing 5 μg/mL cisplatin. The cell proliferation activity as well the expression of apoptosis genes, migration genes and invasion genes was measured. Results: 8 h, 16 h and 24 h after different conditions of treatment, the cell proliferation activity of cisplatin group, Par-4 group and cisplatin + Par-4 group were significantly lower than that of control group, and the cell proliferation activity of cisplatin + Par-4 group was significantly lower than that of cisplatin group and Par-4 group;24 h after different conditions of treatment, Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin group, Par-4 group and cisplatin + Par-4 group were greatly higher than those in control group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly lower than those in control group;Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin + Par-4 group were greatly higher than those in cisplatin group and Par-4 group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly significantly lower than those in cisplatin group and Par-4 group. Conclusion:Par-4 gene overexpression can increase the nephroblastoma sensitivity to cisplatin, reduce cell proliferation activity, promote apoptosis and inhibit cell migration and invasion.展开更多
AIM: To compare apparent diffusion coefficient (ADC) values on diffusion-weighted imaging (DWI) of hepatic fibrosis patients with those of healthy controls and to identify their correlations with serum indices of live...AIM: To compare apparent diffusion coefficient (ADC) values on diffusion-weighted imaging (DWI) of hepatic fibrosis patients with those of healthy controls and to identify their correlations with serum indices of liver fibrosis.展开更多
Objective： The aim of our study was to analysis the pictures of conventional magnetic resonance imaging （MR）, diffusion-weighted imaging （DWl） and dynamic enhanced magnetic resonance imaging （DEMRI） of pancreat...Objective： The aim of our study was to analysis the pictures of conventional magnetic resonance imaging （MR）, diffusion-weighted imaging （DWl） and dynamic enhanced magnetic resonance imaging （DEMRI） of pancreatic endocrine tumors （PETs）, and evaluate diagnostic value of MR, DWl and DEMRI for diagnosing PETs. Methods： DWl and DEMRI scanning toward 13 patients with PETs being confirmed by surgical pathology before surgery on the basis of conventional MR scanning were carried out, and MR findings was analyzed retrospectively. Results： Of 13 patients with PETs there was 11 cases with single lesion, 2 with multiple, and had 15 lesions altogether, of which there were 3 lesions in pancreatic head, 1 in its neck, 2 in its body, 4 in its body and tail, 5 in its tail. MR findings： （1） T1WI signal was low or slightly lower （9/15）, and equal ones （5/15）; （2） T2WI showed high or slightly higher signal （10/15）, and equal ones （5/15）; （3） T1WI with fat suppression： the signal was low （11/15）, mixed signal （2/15）, and equal ones （2/15）; （4） DWI： normal pancreatic tissue exhibited homogeneous intermediate signal, all 15 lesions were high or slightly higher signal, the measured ADC values of tissue of PETs was （1.124 ± 0.252） × 104 mm2/s, and the ADC value of normal pancreatic tissue （1.873 ± 0.157） × 10^3 mm2/s; （5） Enhanced （M3D/LAVA） scanning： among 13 patients with PETs there were 12 pancreatic lesions with significantly enhanced signals in the arterial phase in all 15, and significantly higher than normal pancreatic tissue, and two slight enhancement was slightly higher signal; and 1 no enhancement. Enhanced pattern： homogeneous enhancement were 6 lesions, and the heterogeneous 4, and the edge ring 5. Conclusion： MR and DWl combining with DEMRI help qualitative diagnosis of pancreatic endocrine tumors.展开更多
Objective:To study the correlation of immune response and inflammatory response with human cartilage glycoprotein 39 (YKL-40) content in children with recurrent pneumonia. Methods: Children who were treated in the Cen...Objective:To study the correlation of immune response and inflammatory response with human cartilage glycoprotein 39 (YKL-40) content in children with recurrent pneumonia. Methods: Children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to recurrent pneumonia between May 2014 and October 2017 were selected as the experimental group A of the research, children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to acute pneumonia during the same period were selected as the experimental group B of the study, and children who received physical examination in the Central Hospital of Enshi Autonomous Prefecture during the same period were selected as the control group. Serum was collected to determine the contents of YKL-40, immune molecules and inflammatory molecules, and peripheral blood was collected to determine the expression of immune molecules and inflammatory molecules.Results: Serum YKL-40, IFN-γ, HMGB1, TNF-α, MCP4 and CysLTs levels as well as peripheral blood TLR4 and NF-κB expression intensity of experiment group A and experiment group B were higher than those of control group whereas peripheral blood TIM1, TIM3 and CD19 expression intensity as well as serum IL-4 levels were lower than those of control group, and the change of above indexes in experimental group A was more significant than that in experimental group B;peripheral blood TIM1, TIM3 and CD19 expression intensity as well as serum IL-4 level of experimental group A with high YKL-40 were significantly lower than those of experimental group A with low YKL-40 whereas serum IFN-γ, HMGB1, TNF-α, MCP4 and CysLTs levels as well as peripheral blood TLR4 and NF-κB expression intensity were significantly higher than those of experimental group A with low YKL-40.Conclusions: The abnormal secretion of YKL-40 in children with recurrent pneumonia is closely related to the disorder of immune response and the exceissive activation of inflammatory response.展开更多
Objective: To study the effect of adjuvant interferon aerosol inhalation on inflammatory response and stress response in neonatal viral pneumonia. Methods: The newborns with viral pneumonia who were treated in our hos...Objective: To study the effect of adjuvant interferon aerosol inhalation on inflammatory response and stress response in neonatal viral pneumonia. Methods: The newborns with viral pneumonia who were treated in our hospital between May 2015 and October 2017 were selected as the research subjects and randomly divided into the IFN group who received interferon inhalation combined with routine symptomatic treatment and the control group who received routine symptomatic treatment The contents of inflammatory cytokines and stress mediators in serum as well as the expression of inflammatory signaling molecules in peripheral blood were measured before treatment and 7 d after treatment. Results: Compared with those of same group before treatment, SP-A, sICAM1, suPAR, sTREM1, Copeptin, Ins, NE and 8-iso-PG levels in serum as well as Tim1, Tim3, TLR2, TLR4 and NF-κB mRNA expression in peripheral blood of both groups significantly decreased 7 d after treatment, and SP-A, sICAM1, suPAR, sTREM1, Copeptin, Ins, NE and 8-iso-PG levels in serum as well as Tim1, Tim3, TLR2, TLR4 and NF-κB mRNA expression in peripheral blood of IFN group 7 d after treatment were significantly lower than those of control group. Conclusion: Adjuvant interferon aerosol inhalation can reduce the activation of inflammatory response and stress response in neonatal viral pneumonia.展开更多
Objective:To investigate the expressions of FZD10 and its effect on gastric cancer cells. Methods: The expression of FZD10 in gastric cancer tissues and adjacent cancer tissues was detected by immunohistochemistry;The...Objective:To investigate the expressions of FZD10 and its effect on gastric cancer cells. Methods: The expression of FZD10 in gastric cancer tissues and adjacent cancer tissues was detected by immunohistochemistry;The gastric cancer cell lines AGS and MGC-803 were transfected with siRNA, and then use Western-blot to analysis the transfection efficiency;The proliferation and invasion ability of gastric cancer cell was analyzed by CCK-8 and wound heal.Results: The expression of FZD10 in gastric cancer was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group, the proliferation and cell invasion ability of siRNA transfected with AGS and MGC-803 decreased significantly(P<0.05), while the blank control group and empty control group No significant difference between the negative control groups (P>0.05).Conclusion: The expressions of FZD10 significantly up-regulated in gastric cancer, and silencing its expression will affect the proliferation and invasive ability of gastric cancer cells.展开更多
Objective:To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma.Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into ...Objective:To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma.Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA and NC siRNA respectively. 24 h after siRNA transfection, the RNA in the cells was extracted, and fluorescence quantitative PCR reaction was used to detect the mRNA expression of pro-proliferation genes YB-1, CyclinD1, CDK4, Aurora-A and Ki-67, anti-proliferation genes LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 as well as invasion genes KLF4, RIPK3, HIF-1α, Integrinβ1 and MMP9.Results: YB-1, CyclinD1, CDK4, Aurora-A, Ki-67, KLF4, RIPK3, HIF-1α, Integrinβ1 and MMP9 mRNA expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA group whereas LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 mRNA expression were significantly higher than those in NC-siRNA group.Conclusions: Inhibition of the UBE2C gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder cell proliferation and invasion.展开更多
Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital ...Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study, and the lesion tissue was collected;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study, and the normal adrenal gland tissue was collected. The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migration molecules in clinical tissue samples were determined. Results: The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1, AMF, GPx1, L1CAM, Nrg1, RANK, RANKL, Inβ1, MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group, negatively correlated with the protein expression KLF4, and positively correlated with the protein expression of UBE2C. Conclusion: The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhesion and migration of tumor cells.展开更多
Objective: To investigate the effects of oxaliplatin, leucovorin and fluorouracil on serum tumor markers, VEGF, CRP and matrix metalloproteinases in patients with advanced esophageal cancer. Methods: From March 2012 t...Objective: To investigate the effects of oxaliplatin, leucovorin and fluorouracil on serum tumor markers, VEGF, CRP and matrix metalloproteinases in patients with advanced esophageal cancer. Methods: From March 2012 to March 2017 a total of 248 patients with advanced esophageal cancer were selected as the study subjects. According to random data table, they were divided into control group (n=123) and observation group (n=125) according to random data table. The control group was treated with cisplatin combined with fluorouracil, leucovorin chemotherapy, and patients in the observation group received oxaliplatin, leucovorin and fluorouracil chemotherapy, all patients were treated for 2 cycles. The changes of serum tumor markers, VEGF, CRP and matrix metalloproteinase levels in the two groups before and after treatment was compared. Results: Before treatment, there was no significant difference of the levels of serum CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 between the control group and the observation group. Compared with the group before treatment, the levels of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the two groups were significantly lower. After treatment, the level of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the observation group was significantly lower than those of the control group. Conclusion:Oxaliplatin, leucovorin and fluorouracil chemotherapy can effectively reduce the levels of serum tumor markers, VEGF, CRP and matrix metalloproteinase in patients with advanced esophageal cancer, it has important clinical value.展开更多
Objective:To analyze the renal function and oxidative stress after alprostadil combined with valsartan treatment of early diabetic nephropathy.Methods:A total of 230 patients with early diabetic nephropathy who were t...Objective:To analyze the renal function and oxidative stress after alprostadil combined with valsartan treatment of early diabetic nephropathy.Methods:A total of 230 patients with early diabetic nephropathy who were treated in our hospital between December 2011 and December 2015 were divided into control group (n=115) and observation group (n=115) according to random number table. Control group received valsartan treatment, observation group received alprostadil combined with valsartan treatment, and both lasted for one month. After treatment, renal blood flow parameters were evaluated by ultrasound contrast technique, renal function index levels in peripheral blood and urine were measured, and serum levels of oxidative stress indexes were detected.Results:After 1 month of treatment, area under curve (AUC) and time to peak (TTP) levels under ultrasound contrast of observation group were lower than those of control group while derived peak intensity (DPI) level was higher than that of control group;peripheral blood urea nitrogen (BUN) and serum creatinine (Scr) levels of observation group were lower than those of control group, albumin (Alb) level was higher than that of control group, and urine red blood cell (RBC) level was lower than that of control group;serum oxidative stress indexes malondialdehyde (MDA) and advanced oxidation protein products (AOPP) levels of observation group were lower than those of control group while superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) levels were higher than those of control group.Conclusion: Alprostadil combined with valsartan can optimize renal function and reduce systemic oxidative stress in patients with early diabetic nephropathy.展开更多
AIM: To detect the pathogenetic mutations responsible for nonsyndromic autosomal recessive retinitis pigmentosa(RP) in 2 nonconsanguineous Chinese families. METHODS: The clinical data, including detailed medical histo...AIM: To detect the pathogenetic mutations responsible for nonsyndromic autosomal recessive retinitis pigmentosa(RP) in 2 nonconsanguineous Chinese families. METHODS: The clinical data, including detailed medical history, best corrected visual acuity(BCVA), slit-lamp biomicroscope examination, fundus photography, optical coherence tomography, static perimetry, and full field electroretinogram, were collected from the members of 2 nonconsanguineous Chinese families preliminarily diagnosed with RP. Genomic DNA was extracted from the probands and other available family members;wholeexome sequencing was conducted with the DNA samples provided by the probands, and all mutations detected by whole-exome sequencing were verified using Sanger sequencing in the probands and the other available family members. The verified novel mutations were further sequenced in 192 ethnicity matched healthy controls.RESULTS: The patients from the 2 families exhibited the typical symptoms of RP, including night blindness and progressive constriction of the visual field, and the fundus examinations showed attenuated retinal arterioles, peripheral bone spicule pigment deposits, and waxy optic discs. Whole-exome sequencing revealed a novel nonsense mutation in FAM161 A(c.943 A>T, p.Lys315*) and compound heterozygous mutations in RP1 L1(c.56 C>A, p.Pro19 His;c.5470 C>T, p.Gln1824*). The nonsense c.5470 C>T, p.Gln1824* mutation was novel. All mutations were verified by Sanger sequencing. The mutation p.Lys315* in FAM161A co-segregated with the phenotype, and all the nonsense mutations were absent from the ethnicity matched healthy controls and all available databases.CONCLUSION: We identify 2 novel mutations in genes responsible for autosomal recessive RP, and the mutation in FAM161A is reported for the first time in a Chinese population. Our result not only enriches the knowledge of the mutation frequency and spectrum in the genes responsible for nonsyndromic RP but also provides a new target for future gene therapy.展开更多
AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM...AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM） and 96 normal controls were recruited.Sanger sequencing or clone sequencing were used for mutation screening.Further analyses of the available family members and the 96 normal controls were subsequently conducted to obtain additional evidence of the pathogenicity of these variants.The initial diagnosis of the probands was eo HM.We performed a further comprehensive examination of the available family members after mutations were detected in TRPM1 or NYX. RESULTS： Two novel compound heterozygous mutations in TRPM1 were detected in the recruited families.The proband in family A with eo HM carried a c.2594 C 〉T missense mutation in exon 19 and a c.669 ＋3_669 ＋6del AAGT splicing mutation,which was co-segregated with CSNB1 in this family.A patient in family B with a compound heterozygous missense mutation（c.3262 G〉A and c.3250 T〉C） was detected.No mutations were found in NYX.These two identified compound heterozygous mutations were not found in the 96 normal controls.After further examination of the family members,the patients in family A could be diagnosed as eo HM with CSNB1.However due to the limited clinic data,the patient in family B cloud not clearly diagnosed as CSNB1.CONCLUSION： This study has expanded the mutation spectrum of TRPM1 for CSNB1 and additional studiesare needed to elucidate the association between isolated high myopia and TRPM1 and NYX.展开更多
Diabetic cardiomyopathy(DCM)is one of the major heart complications of diabetic patients.Hydrogen sulfide(H2S)is now recognized as an important signaling molecule and has been shown to attenuate the development of dia...Diabetic cardiomyopathy(DCM)is one of the major heart complications of diabetic patients.Hydrogen sulfide(H2S)is now recognized as an important signaling molecule and has been shown to attenuate the development of diabetic cardiomyopathy.However,the underlying mechanisms linking H2S and the development of DCM have not been fully elucidated.In the present study,we therefore sought to explore the role and mechanism of H2S in the pathogenesis of DCM by establishing high glucose-induced injury model in neonatal rat cardiomyocytes(NRCMs)and H9c2 cells.Using cystathionine gamma-lyase(CSE)overexpression and CSE interference vectors transfection,the cell viability,cell apoptosis,and oxidative stress were determined and compared between the treatment of high glucose induction and exgenous NaHS administration.Meanwhile,the relationship between the CSE/H2S system and Wnt/beta-catenin pathway was analyzed and discussed in the high glucose-induced cardiomyocytes.Our results indicated that H2S played an important protective role in high glucose-induced apoptosis and oxidative stress in cardiomyocytes,as shown by the decreased reactive oxygen species and malondialdehyde levels,and the increased activities of superoxide dismutase,catalase and glutathione peroxidase.Moreover,H:S could attenuate the Wnt/p-catenin signalling pathway and up-regulate the expression of haem oxygenase-1(HO-1)and NAD(P)H:quinone oxidoreductase 1(NQOl)in the diabetic myocardium cells.Together,these results demonstrated that H2S could attenuate high glucoseinduced myocardial injury in rat cardiomyocytes by suppressing Wnt/p-catenin pathway.展开更多
文摘Objective:The aim was to evaluate the role of whole body diffusion weighted imaging(WB-DWI) of magnetic resonance in the diagnosis and efficacy evaluation of malignant lymphoma.Methods:The clinical manifestation of whole body diffusion weighted imaging of 47 patients with malignant lymphoma being pathologically proved were carried on by retrospective analysis and to compare with 10 healthy volunteers.There were 8 cases of Hodgkin's lymphoma(HD) patients,of which WB-DWI examination of 5 cases were carried out before and after treatment and 3 cases were done after treatment.There were 39 cases of non-Hodgkin's lymphoma(NHL) patients,of which WB-DWI examination in 19 cases were acted before treatment,11 cases were done before and after treatment and 9 cases done after treatment.In apparent diffusion coefficient(ADC) diagram the ADC values of lymph nodes in patients with malignant lymphoma and healthy volunteers were measured respectively,among 16 patients the ADC values of parts of lymph node being consistent with the initial inspection were determined again and compared the values before and after,and compared with healthy volunteers.Results:Whole body magnetic resonance diffusion weighted imaging(MR-DWI) showed more sensitive to lymphoma,and 372 lymph nodes greater than 1 cm were detected with MR-DWI.Before treatment,mean ADC value of 35 patients with malignant lymphoma was(0.86 ± 0.21) × 10-3 mm2/s,of which an average ADC values of 28 cases after treatment was(1.22 ± 0.31) × 10-3 mm2/s,before and after treatment difference of the average ADC values of patients was statistically significant(P < 0.05);the average ADC value of cervical lymph nodes of 10 healthy volunteers(1.29 ± 0.12) × 10-3 mm2/s and of 30 patients with malignant lymphoma before treatment was statistically significant(P < 0.05),and to compare with 28 patients after treatment difference of the ADC values was not statistically significant(P > 0.05).Conclusion:WB-DWI and ADC values being measured in the clinical diagnosis of malignant lymphoma,staging and evaluation of efficacy of monitoring is a fast and effective technology,with some clinical value.
文摘Objective:To study the effect of hemodialysis (HD) combined with hemoperfusion (HP) on dialysis efficiency, lipid metabolism and atherosclerosis in patients with uremia.Methods:A total of 70 patients with uremia who were treated in our hospital between March 2013 and October 2015 were collected and divided into observation group and control group (n=35) according to double-blind randomized control method. Observation group of patients received hemodialysis combined with hemoperfusion, control group of patients received hemodialysis alone, and the treatment lasted for 6 months. After 6 months of intervention, automatic biochemical analyzer was used to detect renal function indexes and lipid metabolism indexes, and the two-dimensional ultrasound was used to quantitatively determine the carotid atherosclerosis parameters.Results:Before intervention, differences in renal function, lipid metabolism and atherosclerosis levels were not statistically significant between two groups of patients;after 6 months of intervention, renal function indexes blood urea nitrogen (BUN), serum creatinine (Scr),β2-microglobulin (β2-MG) and blood uric acid (BUA) levels of observation group were lower than those of control group, lipid metabolism indexes total cholesterol (TC), triglyceride (TG), apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) levels were lower than those of control group, and differences between groups were statistically significant;quantitative carotid ultrasound parameters gray-scale median (GSM) of observation group was higher than that of control group, enhanced intensity (EI) and enhanced density (ED) were lower than those of control group, and differences between groups were statistically significant.Conclusion:Hemodialysis combined with hemoperfusion can improve the dialysis efficiency, also reduce lipid metabolism disturbance and delay the formation of atherosclerosis in patients with uremia.
文摘Objective:To study the correlation of Doppler ultrasound parameters with angiogenesis and cancer cell growth in endometrial cancer.Methods: Patients with endometrial cancer and fibroids who accepted surgical resection in the Central Hospital of Enshi Autonomous Prefecture between March 2015 and June 2017 were selected as the research subjects and enrolled in the observation group and control group of the study respectively. The preoperative pelvic Doppler ultrasonography was done to determine the blood flow parameters resistance index (RI) and pulsatility index (PI) of the endometrial lesions;after surgical resection, the endometrial cancer lesion tissue was collected from the observation group and normal endometrial tissue was collected from the control group to determine the contents of pro-proliferation molecules, pro-invasion molecules and angiogenesis molecules.Results: PI and RI levels in endometrial cancer lesion of observation group were significantly lower than those of control group, and cIAP1, cIAP2, RLIP76, p-Pak4ser474, EFEMP2, CXCR4, CXCR7, Snail, Septin-9,β-arrestin2 and SOX2 protein levels in endometrial cancer lesion were significantly higher than those of control group;PI and RI levels in endometrial cancer lesion were negatively correlated with cIAP1, cIAP2, RLIP76, p-Pak4ser474, EFEMP2, CXCR4, CXCR7, Snail, Septin-9,β-arrestin2 and SOX2 protein levels.Conclusion: The Doppler ultrasound parameters of endometrial carcinoma lesion are closely related to the angiogenesis and cancer cell growth in the lesion.
文摘Objective: To study the effect of trastuzumab combined with paclitaxel neoadjuvant chemotherapy on the cell proliferation and invasion in human epidermal growth factor receptor-2 (HER-2)-positive breast cancer lesions. Methods: Patients who were diagnosed with HER-2-positive breast cancer in the Central Hospital of Enshi Autonomous Prefecture Hubei Province between April 2015 and January 2017 were selected and randomly divided into two groups, the combined group received trastuzumab combined with paclitaxel chemotherapy, and the control group accepted paclitaxel chemotherapy. The surgically removed breast cancer lesions were collected to determine the expression of cell proliferation genes, cell invasion genes and angiogenesis molecules. Results: USP39, EphA2, NUAK1, Gab2, Raptor, ICAM-1, HIF-1α, VEGF, ANGPLT-2 and ANGPLT-4 mRNA expression in tumor lesion of combined group were significantly lower than those of control group while CCN5, ALEX1, ATG2B, ATG4D, E-cadherin and EBP50 mRNA expression were significantly higher than those of control group. Conclusion: Trastuzumab combined with paclitaxel neoadjuvant chemotherapy can inhibit the cell proliferation and invasion and decrease the angiogenesis in HER-2-positive breast cancer lesions.
文摘Objective:To study the evaluation value of HMGA2, Tiam1 and Notch1 expression for cancer cell proliferation, invasion and epithelial-mesenchymal transition in non-small cell lung cancer tissue. Methods:Non-small cell lung cancer tissues and para-carcinoma tissues surgically removed in our hospital between May 2013 and December 2015 were collected, immunohistochemical staining method was used to determine HMGA2, Tiam1 and Notch1 protein expression, and fluorescence quantitative PCR was used to determine the mRNA expression of HMGA2, Tiam1, Notch1 as well as proliferation, invasion and epithelial-mesenchymal transition molecules. Results:HMGA2, Tiam1 and Notch1 mRNA expression and positive protein expression rate in lung cancer tissues were significantly higher than those in para-carcinoma tissues (P<0.05);E-cadherin mRNA expression in lung cancer tissues with positive HMGA2 expression was significantly lower than that in lung cancer tissues with negative HMGA2 expression (P<0.05) while N-cadherin and Vimentin mRNA expression were significantly higher than those in lung cancer tissues with negative HMGA2 expression (P<0.05);MMP2, MMP3, MMP8 and MMP9 mRNA expression in lung cancer tissues with positive Tiam1 expression were significantly higher than those in lung cancer tissues with negative Tiam1 expression (P<0.05);CyclinD1, Bcl-2 and Survivin mRNA expression in lung cancer tissues with positive Notch1 expression were significantly higher those in lung cancer tissues with negative Notch1 expression (P<0.05). Conclusions:Highly expressed HMGA2, Tiam1 and Notch1 in lung cancer tissues can regulate the epithelial-mesenchymal transition, invasion and proliferation of cancer cells respectively.
文摘Objective: To measure the contrast-enhanced ultrasound parameters of endometrial benign and malignant lesions and investigate their correlation with malignant molecule expression. Methods: A total of 118 patients who were examined and diagnosed with endometrial benign hyperplasia in the hospital between December 2012 and January 2017 were collected as endometrial hyperplasia group, and 67 patients with endometrial carcinoma were collected as endometrial cancer group. The differences in the levels of uterine contrast-enhanced ultrasound parameters as well as the expression of proliferation, apoptosis and invasion genes in endometrial lesion tissue were compared between the two groups. Pearson test was used to evaluate the correlation between uterine contrast-enhanced ultrasound parameters and malignant molecule expression in the lesions. Results: Contrast-enhanced ultrasound parameter TTP level of endometrial cancer group was lower than that of endometrial hyperplasia group whereas PI level was higher than that of endometrial hyperplasia group. Proliferation gene EFEMP2 mRNA expression in lesion tissue of endometrial cancer group was lower than that of endometrial hyperplasia group whereas RRM2 and DJ-1 mRNA expression were higher than those of endometrial hyperplasia group;apoptosis genes P53, c-myc and Livin mRNA expression were higher than those of endometrial hyperplasia group whereas Caspase-3 and Bid mRNA expression were lower than those of endometrial hyperplasia group;invasion gene DKK1 mRNA expression was lower than that of endometrial hyperplasia group whereas HMGB1, EZH2 and Snail mRNA expression were higher than those of endometrial hyperplasia group. Pearson test showed that the contrast-enhanced ultrasound parameter levels of endometrial cancer were directly correlated with the malignant molecule expression in the lesion tissues. Conclusion: contrast-enhanced ultrasound parameter levels can be used to identify endometrial benign and malignant lesions and are directly correlated with the severity of the lesion.
文摘Objective: To study the regulating effect of Par-4 gene overexpression on the nephroblastoma sensitivity to cisplatin. Methods: Nephroblastoma SK-NEP-1 cells were cultured and divided into four groups, control group were treated with RMPI-1640 without serum or drugs, cisplatin group were treated with serum-free RMPI-1640 containing 5 μg/mL cisplatin, Par-4 group were transfected by Par-4 overexpression plasmids with serum-free RMPI-1640, and cisplatin+ Par-4 group were transfected by Par-4 overexpression plasmid with serum-free RMPI-1640 containing 5 μg/mL cisplatin. The cell proliferation activity as well the expression of apoptosis genes, migration genes and invasion genes was measured. Results: 8 h, 16 h and 24 h after different conditions of treatment, the cell proliferation activity of cisplatin group, Par-4 group and cisplatin + Par-4 group were significantly lower than that of control group, and the cell proliferation activity of cisplatin + Par-4 group was significantly lower than that of cisplatin group and Par-4 group;24 h after different conditions of treatment, Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin group, Par-4 group and cisplatin + Par-4 group were greatly higher than those in control group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly lower than those in control group;Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin + Par-4 group were greatly higher than those in cisplatin group and Par-4 group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly significantly lower than those in cisplatin group and Par-4 group. Conclusion:Par-4 gene overexpression can increase the nephroblastoma sensitivity to cisplatin, reduce cell proliferation activity, promote apoptosis and inhibit cell migration and invasion.
文摘AIM: To compare apparent diffusion coefficient (ADC) values on diffusion-weighted imaging (DWI) of hepatic fibrosis patients with those of healthy controls and to identify their correlations with serum indices of liver fibrosis.
文摘Objective： The aim of our study was to analysis the pictures of conventional magnetic resonance imaging （MR）, diffusion-weighted imaging （DWl） and dynamic enhanced magnetic resonance imaging （DEMRI） of pancreatic endocrine tumors （PETs）, and evaluate diagnostic value of MR, DWl and DEMRI for diagnosing PETs. Methods： DWl and DEMRI scanning toward 13 patients with PETs being confirmed by surgical pathology before surgery on the basis of conventional MR scanning were carried out, and MR findings was analyzed retrospectively. Results： Of 13 patients with PETs there was 11 cases with single lesion, 2 with multiple, and had 15 lesions altogether, of which there were 3 lesions in pancreatic head, 1 in its neck, 2 in its body, 4 in its body and tail, 5 in its tail. MR findings： （1） T1WI signal was low or slightly lower （9/15）, and equal ones （5/15）; （2） T2WI showed high or slightly higher signal （10/15）, and equal ones （5/15）; （3） T1WI with fat suppression： the signal was low （11/15）, mixed signal （2/15）, and equal ones （2/15）; （4） DWI： normal pancreatic tissue exhibited homogeneous intermediate signal, all 15 lesions were high or slightly higher signal, the measured ADC values of tissue of PETs was （1.124 ± 0.252） × 104 mm2/s, and the ADC value of normal pancreatic tissue （1.873 ± 0.157） × 10^3 mm2/s; （5） Enhanced （M3D/LAVA） scanning： among 13 patients with PETs there were 12 pancreatic lesions with significantly enhanced signals in the arterial phase in all 15, and significantly higher than normal pancreatic tissue, and two slight enhancement was slightly higher signal; and 1 no enhancement. Enhanced pattern： homogeneous enhancement were 6 lesions, and the heterogeneous 4, and the edge ring 5. Conclusion： MR and DWl combining with DEMRI help qualitative diagnosis of pancreatic endocrine tumors.
文摘Objective:To study the correlation of immune response and inflammatory response with human cartilage glycoprotein 39 (YKL-40) content in children with recurrent pneumonia. Methods: Children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to recurrent pneumonia between May 2014 and October 2017 were selected as the experimental group A of the research, children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to acute pneumonia during the same period were selected as the experimental group B of the study, and children who received physical examination in the Central Hospital of Enshi Autonomous Prefecture during the same period were selected as the control group. Serum was collected to determine the contents of YKL-40, immune molecules and inflammatory molecules, and peripheral blood was collected to determine the expression of immune molecules and inflammatory molecules.Results: Serum YKL-40, IFN-γ, HMGB1, TNF-α, MCP4 and CysLTs levels as well as peripheral blood TLR4 and NF-κB expression intensity of experiment group A and experiment group B were higher than those of control group whereas peripheral blood TIM1, TIM3 and CD19 expression intensity as well as serum IL-4 levels were lower than those of control group, and the change of above indexes in experimental group A was more significant than that in experimental group B;peripheral blood TIM1, TIM3 and CD19 expression intensity as well as serum IL-4 level of experimental group A with high YKL-40 were significantly lower than those of experimental group A with low YKL-40 whereas serum IFN-γ, HMGB1, TNF-α, MCP4 and CysLTs levels as well as peripheral blood TLR4 and NF-κB expression intensity were significantly higher than those of experimental group A with low YKL-40.Conclusions: The abnormal secretion of YKL-40 in children with recurrent pneumonia is closely related to the disorder of immune response and the exceissive activation of inflammatory response.
文摘Objective: To study the effect of adjuvant interferon aerosol inhalation on inflammatory response and stress response in neonatal viral pneumonia. Methods: The newborns with viral pneumonia who were treated in our hospital between May 2015 and October 2017 were selected as the research subjects and randomly divided into the IFN group who received interferon inhalation combined with routine symptomatic treatment and the control group who received routine symptomatic treatment The contents of inflammatory cytokines and stress mediators in serum as well as the expression of inflammatory signaling molecules in peripheral blood were measured before treatment and 7 d after treatment. Results: Compared with those of same group before treatment, SP-A, sICAM1, suPAR, sTREM1, Copeptin, Ins, NE and 8-iso-PG levels in serum as well as Tim1, Tim3, TLR2, TLR4 and NF-κB mRNA expression in peripheral blood of both groups significantly decreased 7 d after treatment, and SP-A, sICAM1, suPAR, sTREM1, Copeptin, Ins, NE and 8-iso-PG levels in serum as well as Tim1, Tim3, TLR2, TLR4 and NF-κB mRNA expression in peripheral blood of IFN group 7 d after treatment were significantly lower than those of control group. Conclusion: Adjuvant interferon aerosol inhalation can reduce the activation of inflammatory response and stress response in neonatal viral pneumonia.
基金Natural Science Foundation of Hubei Province.Project No:2016CFB668.
文摘Objective:To investigate the expressions of FZD10 and its effect on gastric cancer cells. Methods: The expression of FZD10 in gastric cancer tissues and adjacent cancer tissues was detected by immunohistochemistry;The gastric cancer cell lines AGS and MGC-803 were transfected with siRNA, and then use Western-blot to analysis the transfection efficiency;The proliferation and invasion ability of gastric cancer cell was analyzed by CCK-8 and wound heal.Results: The expression of FZD10 in gastric cancer was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group, the proliferation and cell invasion ability of siRNA transfected with AGS and MGC-803 decreased significantly(P<0.05), while the blank control group and empty control group No significant difference between the negative control groups (P>0.05).Conclusion: The expressions of FZD10 significantly up-regulated in gastric cancer, and silencing its expression will affect the proliferation and invasive ability of gastric cancer cells.
文摘Objective:To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma.Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA and NC siRNA respectively. 24 h after siRNA transfection, the RNA in the cells was extracted, and fluorescence quantitative PCR reaction was used to detect the mRNA expression of pro-proliferation genes YB-1, CyclinD1, CDK4, Aurora-A and Ki-67, anti-proliferation genes LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 as well as invasion genes KLF4, RIPK3, HIF-1α, Integrinβ1 and MMP9.Results: YB-1, CyclinD1, CDK4, Aurora-A, Ki-67, KLF4, RIPK3, HIF-1α, Integrinβ1 and MMP9 mRNA expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA group whereas LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 mRNA expression were significantly higher than those in NC-siRNA group.Conclusions: Inhibition of the UBE2C gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder cell proliferation and invasion.
文摘Objective: To study the correlation of KLF4 and UBE2C expression levels in neuroblastoma with cell adhesion and migration. Methods: A total of 56 children who were diagnosed with neuroblastoma in the Central Hospital of Enshi Autonomous Prefecture between May 2014 and February 2017 were selected as the NB group of the study, and the lesion tissue was collected;38 children who were treated in the Central Hospital of Enshi Autonomous Prefecture due to serious hydronephrosis during the same period were selected as the control group of the study, and the normal adrenal gland tissue was collected. The mRNA expression and protein expression of KLF4 and UBE2C as well as the protein expression of cell adhesion molecules and migration molecules in clinical tissue samples were determined. Results: The mRNA expression and protein expression of KLF4 in neuroblastoma tissue of NB group were greatly lower than those of control group whereas the mRNA expression and protein expression of UBE2C were greatly higher than those of control group;PDLIM1, AMF, GPx1, L1CAM, Nrg1, RANK, RANKL, Inβ1, MTA1 and MMP9 protein expression in neuroblastoma tissue of NB group were greatly higher than those of control group, negatively correlated with the protein expression KLF4, and positively correlated with the protein expression of UBE2C. Conclusion: The low expression of KLF4 and the high expression of UBE2C in neuroblastoma can promote the adhesion and migration of tumor cells.
文摘Objective: To investigate the effects of oxaliplatin, leucovorin and fluorouracil on serum tumor markers, VEGF, CRP and matrix metalloproteinases in patients with advanced esophageal cancer. Methods: From March 2012 to March 2017 a total of 248 patients with advanced esophageal cancer were selected as the study subjects. According to random data table, they were divided into control group (n=123) and observation group (n=125) according to random data table. The control group was treated with cisplatin combined with fluorouracil, leucovorin chemotherapy, and patients in the observation group received oxaliplatin, leucovorin and fluorouracil chemotherapy, all patients were treated for 2 cycles. The changes of serum tumor markers, VEGF, CRP and matrix metalloproteinase levels in the two groups before and after treatment was compared. Results: Before treatment, there was no significant difference of the levels of serum CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 between the control group and the observation group. Compared with the group before treatment, the levels of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the two groups were significantly lower. After treatment, the level of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the observation group was significantly lower than those of the control group. Conclusion:Oxaliplatin, leucovorin and fluorouracil chemotherapy can effectively reduce the levels of serum tumor markers, VEGF, CRP and matrix metalloproteinase in patients with advanced esophageal cancer, it has important clinical value.
文摘Objective:To analyze the renal function and oxidative stress after alprostadil combined with valsartan treatment of early diabetic nephropathy.Methods:A total of 230 patients with early diabetic nephropathy who were treated in our hospital between December 2011 and December 2015 were divided into control group (n=115) and observation group (n=115) according to random number table. Control group received valsartan treatment, observation group received alprostadil combined with valsartan treatment, and both lasted for one month. After treatment, renal blood flow parameters were evaluated by ultrasound contrast technique, renal function index levels in peripheral blood and urine were measured, and serum levels of oxidative stress indexes were detected.Results:After 1 month of treatment, area under curve (AUC) and time to peak (TTP) levels under ultrasound contrast of observation group were lower than those of control group while derived peak intensity (DPI) level was higher than that of control group;peripheral blood urea nitrogen (BUN) and serum creatinine (Scr) levels of observation group were lower than those of control group, albumin (Alb) level was higher than that of control group, and urine red blood cell (RBC) level was lower than that of control group;serum oxidative stress indexes malondialdehyde (MDA) and advanced oxidation protein products (AOPP) levels of observation group were lower than those of control group while superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) levels were higher than those of control group.Conclusion: Alprostadil combined with valsartan can optimize renal function and reduce systemic oxidative stress in patients with early diabetic nephropathy.
基金Supported by the National Natural Science Foundation of China(No.81360154)
文摘AIM: To detect the pathogenetic mutations responsible for nonsyndromic autosomal recessive retinitis pigmentosa(RP) in 2 nonconsanguineous Chinese families. METHODS: The clinical data, including detailed medical history, best corrected visual acuity(BCVA), slit-lamp biomicroscope examination, fundus photography, optical coherence tomography, static perimetry, and full field electroretinogram, were collected from the members of 2 nonconsanguineous Chinese families preliminarily diagnosed with RP. Genomic DNA was extracted from the probands and other available family members;wholeexome sequencing was conducted with the DNA samples provided by the probands, and all mutations detected by whole-exome sequencing were verified using Sanger sequencing in the probands and the other available family members. The verified novel mutations were further sequenced in 192 ethnicity matched healthy controls.RESULTS: The patients from the 2 families exhibited the typical symptoms of RP, including night blindness and progressive constriction of the visual field, and the fundus examinations showed attenuated retinal arterioles, peripheral bone spicule pigment deposits, and waxy optic discs. Whole-exome sequencing revealed a novel nonsense mutation in FAM161 A(c.943 A>T, p.Lys315*) and compound heterozygous mutations in RP1 L1(c.56 C>A, p.Pro19 His;c.5470 C>T, p.Gln1824*). The nonsense c.5470 C>T, p.Gln1824* mutation was novel. All mutations were verified by Sanger sequencing. The mutation p.Lys315* in FAM161A co-segregated with the phenotype, and all the nonsense mutations were absent from the ethnicity matched healthy controls and all available databases.CONCLUSION: We identify 2 novel mutations in genes responsible for autosomal recessive RP, and the mutation in FAM161A is reported for the first time in a Chinese population. Our result not only enriches the knowledge of the mutation frequency and spectrum in the genes responsible for nonsyndromic RP but also provides a new target for future gene therapy.
基金Supported by the National Nature Science Foundation of China(No.81362138)
文摘AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM） and 96 normal controls were recruited.Sanger sequencing or clone sequencing were used for mutation screening.Further analyses of the available family members and the 96 normal controls were subsequently conducted to obtain additional evidence of the pathogenicity of these variants.The initial diagnosis of the probands was eo HM.We performed a further comprehensive examination of the available family members after mutations were detected in TRPM1 or NYX. RESULTS： Two novel compound heterozygous mutations in TRPM1 were detected in the recruited families.The proband in family A with eo HM carried a c.2594 C 〉T missense mutation in exon 19 and a c.669 ＋3_669 ＋6del AAGT splicing mutation,which was co-segregated with CSNB1 in this family.A patient in family B with a compound heterozygous missense mutation（c.3262 G〉A and c.3250 T〉C） was detected.No mutations were found in NYX.These two identified compound heterozygous mutations were not found in the 96 normal controls.After further examination of the family members,the patients in family A could be diagnosed as eo HM with CSNB1.However due to the limited clinic data,the patient in family B cloud not clearly diagnosed as CSNB1.CONCLUSION： This study has expanded the mutation spectrum of TRPM1 for CSNB1 and additional studiesare needed to elucidate the association between isolated high myopia and TRPM1 and NYX.
基金the Natural Science Foundation of Hubei Province of China(No.2011CDC146).
文摘Diabetic cardiomyopathy(DCM)is one of the major heart complications of diabetic patients.Hydrogen sulfide(H2S)is now recognized as an important signaling molecule and has been shown to attenuate the development of diabetic cardiomyopathy.However,the underlying mechanisms linking H2S and the development of DCM have not been fully elucidated.In the present study,we therefore sought to explore the role and mechanism of H2S in the pathogenesis of DCM by establishing high glucose-induced injury model in neonatal rat cardiomyocytes(NRCMs)and H9c2 cells.Using cystathionine gamma-lyase(CSE)overexpression and CSE interference vectors transfection,the cell viability,cell apoptosis,and oxidative stress were determined and compared between the treatment of high glucose induction and exgenous NaHS administration.Meanwhile,the relationship between the CSE/H2S system and Wnt/beta-catenin pathway was analyzed and discussed in the high glucose-induced cardiomyocytes.Our results indicated that H2S played an important protective role in high glucose-induced apoptosis and oxidative stress in cardiomyocytes,as shown by the decreased reactive oxygen species and malondialdehyde levels,and the increased activities of superoxide dismutase,catalase and glutathione peroxidase.Moreover,H:S could attenuate the Wnt/p-catenin signalling pathway and up-regulate the expression of haem oxygenase-1(HO-1)and NAD(P)H:quinone oxidoreductase 1(NQOl)in the diabetic myocardium cells.Together,these results demonstrated that H2S could attenuate high glucoseinduced myocardial injury in rat cardiomyocytes by suppressing Wnt/p-catenin pathway.
