Objective： To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela. Methods： According to the seq...Objective： To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela. Methods： According to the sequence of NBS1 mRNA, the NBS1 pre-microRNA was designed and synthesized, then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cells. The integrity of the insert fragment was verified through colony PCR and sequencing analysis. The NBS1 gene expression of NBS1 microRNA recombinants was detected by Real-Time PCR and western blot. Telomerase activity in Hela cells was assayed by TRAP-PCR-EB. Results： Sequences of insert fragment in microRNA expressing recombinants were correct. The NBS1 gene expression was decreased, and the telomerase activation of Hela cell reduced. Conclusion： NBS1 microRNA inhibits NBS1 gene expression, and depresses telomerase activation of Hela cells. This confirms that there is relevance between NBS 1 gene and telomerase activity.展开更多
Collecting massive commonsense knowledge （CSK） for commonsense reasoning has been a long time standing challenge within artificial intelligence research. Numerous methods and systems for acquiring CSK have been deve...Collecting massive commonsense knowledge （CSK） for commonsense reasoning has been a long time standing challenge within artificial intelligence research. Numerous methods and systems for acquiring CSK have been developed to overcome the knowledge acquisition bottleneck. Although some specific commonsense reasoning tasks have been presented to allow researchers to measure and compare the performance of their CSK systems, we compare them at a higher level from the following aspects： CSK acquisition task （what CSK is acquired from where）, technique used （how can CSK be acquired）, and CSK evaluation methods （how to evaluate the acquired CSK）. In this survey, we first present a categorization of CSK acquisition systems and the great challenges in the field. Then, we review and compare the CSK acquisition systems in detail. Finally, we conclude the current progress in this field and explore some promising future research issues.展开更多
文摘Objective： To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela. Methods： According to the sequence of NBS1 mRNA, the NBS1 pre-microRNA was designed and synthesized, then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cells. The integrity of the insert fragment was verified through colony PCR and sequencing analysis. The NBS1 gene expression of NBS1 microRNA recombinants was detected by Real-Time PCR and western blot. Telomerase activity in Hela cells was assayed by TRAP-PCR-EB. Results： Sequences of insert fragment in microRNA expressing recombinants were correct. The NBS1 gene expression was decreased, and the telomerase activation of Hela cell reduced. Conclusion： NBS1 microRNA inhibits NBS1 gene expression, and depresses telomerase activation of Hela cells. This confirms that there is relevance between NBS 1 gene and telomerase activity.
基金supported by the National Natural Science Foundation of China under Grant Nos.91224006,61173063,61035004,61203284,and 309737163the National Social Science Foundation of China under Grant No.10AYY003
文摘Collecting massive commonsense knowledge （CSK） for commonsense reasoning has been a long time standing challenge within artificial intelligence research. Numerous methods and systems for acquiring CSK have been developed to overcome the knowledge acquisition bottleneck. Although some specific commonsense reasoning tasks have been presented to allow researchers to measure and compare the performance of their CSK systems, we compare them at a higher level from the following aspects： CSK acquisition task （what CSK is acquired from where）, technique used （how can CSK be acquired）, and CSK evaluation methods （how to evaluate the acquired CSK）. In this survey, we first present a categorization of CSK acquisition systems and the great challenges in the field. Then, we review and compare the CSK acquisition systems in detail. Finally, we conclude the current progress in this field and explore some promising future research issues.
