Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and m...Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ;thus,the mechanism of Hg(II)transport in the human intestine is still unclear.Here,a gut-on-a-chip integrated with transepithelial electrical resistance(TEER)sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II)in situ.The cellular microenvironment was recreated by applying fluid shear stress(0.02 dyne/cm^(2))and cyclic mechanical strain(1%,0.15 Hz).Hg(II)absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II)mixed in culture medium.Hg(II)absorption increased by 23.59%when tensile strain increased from 1%to 5%,and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.展开更多
When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins ...When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins involved in cellular stress are heat shock proteins （HSPs）.展开更多
A fundamental aspect of cancer development is cancer cell proliferation.Seeking for chemical agents that can interfere with cancer cell growth has been of great interest over the years.In our study,we found that a ben...A fundamental aspect of cancer development is cancer cell proliferation.Seeking for chemical agents that can interfere with cancer cell growth has been of great interest over the years.In our study,we found that a benzoxazine derivative,(6-tert-butyl-3,4-dihydro-2Hbenzo[b][1,4]oxazin-3-yl)methanol(TBM),could inhibit cell growth and caused significant cell cycle arrest in pulmonary adenocarcinoma A549 and H460 cells with wild-type p53,while not affecting the cell cycle distribution in p53-deleted H1299 lung adenocarcinoma cells.Since P53 plays an important role in regulating cell cycle progression,we analyzed the protein level of p53 by Western blot,and detected a significant elevation of p53 level after TBM treatment in A549 and H460 cells.The data suggested that TBM might specifically inhibit the proliferation of p53 wild-type lung adenocarcinoma cells through a p53-dependent cell cycle control pathway.More interestingly,results indicated that TBM might serve as a useful tool for studying the molecular mechanisms of lung cancer cell growth and cell cycle control,especially for the biologic process regulated by P53.展开更多
基金supported by the Taishan Scholars Program of Shandong Province (No.tsqn201812087)the National Natural Science Foundation of China (No.61901239)+1 种基金Qingchuang Science and Technology Plan Foundation for Colleges and Universities in Shandong Province (No.2019KJB009)the Young Ph.D.Cooperation Foundation of Qilu University of Technology (Shandong Academy of Sciences) (No.2019BSHZ002).
文摘Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ;thus,the mechanism of Hg(II)transport in the human intestine is still unclear.Here,a gut-on-a-chip integrated with transepithelial electrical resistance(TEER)sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II)in situ.The cellular microenvironment was recreated by applying fluid shear stress(0.02 dyne/cm^(2))and cyclic mechanical strain(1%,0.15 Hz).Hg(II)absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II)mixed in culture medium.Hg(II)absorption increased by 23.59%when tensile strain increased from 1%to 5%,and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.
基金supported by the National Natural Science Foundation of China (31571321, 31171428)the National Key Research and Develepment Program of China (2016YFE0129200)+1 种基金the Institute of the Fundamental Research Funds of Shandong University (2015JC036)the Open Projects of State Key Laboratory of Molecular Oncology (SKL-KF-2017-17)
文摘When exposing to environmental stress or internal damage, such as genotoxic stress, oxidative stress, and heat stress, cells produce a series of adaptive responses called cellular stress responses. The major proteins involved in cellular stress are heat shock proteins （HSPs）.
基金This study was supported by the National Natural Science Foundation of China(Grant No.90813022)by the Natural Science Foundation of Shandong Province(Nos.Z2008D04 and Z2008B10).
文摘A fundamental aspect of cancer development is cancer cell proliferation.Seeking for chemical agents that can interfere with cancer cell growth has been of great interest over the years.In our study,we found that a benzoxazine derivative,(6-tert-butyl-3,4-dihydro-2Hbenzo[b][1,4]oxazin-3-yl)methanol(TBM),could inhibit cell growth and caused significant cell cycle arrest in pulmonary adenocarcinoma A549 and H460 cells with wild-type p53,while not affecting the cell cycle distribution in p53-deleted H1299 lung adenocarcinoma cells.Since P53 plays an important role in regulating cell cycle progression,we analyzed the protein level of p53 by Western blot,and detected a significant elevation of p53 level after TBM treatment in A549 and H460 cells.The data suggested that TBM might specifically inhibit the proliferation of p53 wild-type lung adenocarcinoma cells through a p53-dependent cell cycle control pathway.More interestingly,results indicated that TBM might serve as a useful tool for studying the molecular mechanisms of lung cancer cell growth and cell cycle control,especially for the biologic process regulated by P53.
