To evaluate the therapeutic effects of bone marrow-derived CD11b<sup>+</sup>CD14<sup>+</sup> monocytes in a murine model of chronic liver damage.METHODSChronic liver damage was induced in C57BL...To evaluate the therapeutic effects of bone marrow-derived CD11b<sup>+</sup>CD14<sup>+</sup> monocytes in a murine model of chronic liver damage.METHODSChronic liver damage was induced in C57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent assay.RESULTSCD11b<sup>+</sup>CD14<sup>+</sup> monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. CD11b<sup>+</sup>CD14<sup>+</sup> monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and osteopontin.CONCLUSIONMonocyte therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.展开更多
Several studies have shown a major development in height and cannon bone circumference in foals from birth to weaning and from different breeds. However, in Brazil, there is little information about foal development. ...Several studies have shown a major development in height and cannon bone circumference in foals from birth to weaning and from different breeds. However, in Brazil, there is little information about foal development. For this reason, this study has used data on foals from seven different farms in Pernambuco, Northeast Brazil, where horses have a major economic importance. The aim of this study was to compare foal’s development from four breeds and to develop a regression formula in order to estimate such development. It was used data from 210 suckling foals (Arabians n = 18, Campolina n = 86, Mangalarga Marchador n = 42 and Quarter Horses n = 64), raised in similar management practices. Their height at withers, heart girth, and cannon bone girth were measured monthly from birth to weaning. Data we reanalyzed by ANOVA and Tukey Test, with P established in 5%. The highest mean of height at withers was observed in the Arabian foals, and it was similar to Campolina and Mangalarga Marchador foals. The last breed presented a higher percentage of gain in height (~36%) and higher heart girth. On the other hand, Campolina foals had the highest mean of cannon bone girth. There was a significant interaction between breed and development phase in heart and cannon bone girths. All regression for mulas obtained from this data was very significant, and it was showed that they can predict height at withers in the four breeds studied. Heart and cannon bone girths showed medium value of R2 and large significance. It is important to highlight that fetal programing produces significant impact on foal development, even after weaning. So, this study would have observed some more changes if its scope had been extended beyond this phase.展开更多
The monitoring and development of foals, based on biometric indices, and the determination of body composition could be important for genetic improvement and the elaboration of nutritional plans for horses. Considerin...The monitoring and development of foals, based on biometric indices, and the determination of body composition could be important for genetic improvement and the elaboration of nutritional plans for horses. Considering that little information was available, the aim of the present study was to assess the growth and determine the body composition of suckling Quarter Horse foals. Animals (n = 46) of both sexes were monitored monthly from birth until the fifth month of age, using an ultrasound device and the following measurements: Wither Height (WH);Chest Perimeter (CP);Cannon Bone Circumference (CBC) Cannon Bone Girth (CG);Body Mass (BM);and Rump Fat Thickness (RFT). These tests determined the Fat-Free Mass (FFM), BM, Fat Percentage (FP) and Fat Thickness (FT). The data were analyzed using ANOVA and Tukey’s test, with differences considered statistically significant when P ≤ 0.05. The results demonstrated that the parameters analyzed changed significantly during the study period. The BM was ~41 at birth and ~177 kg at the point of weaning. Conversely, the FM was ~3.8 at birth and 18.1 kg at the point of weaning. The FFM was ~37 and ~160 kg at the time of birth and weaning, respectively. The FP varied from 9.3 at birth to 9.54 at weaning. The mean FT was ~1.39 and ~1.91 cm at birth and weaning, respectively. The WH measured ~91 at birth and 116 cm at weaning. In conclusion, suckling foals gain BM quickly from the time of birth to the time of weaning. The main cause of this gain is FFM. Therefore, due to the significant changes in the parameters studied herein, it is important to monitor these values in order to prevent nutritional imbalances.展开更多
Dirofilaria immitis is an important nematode parasite commonly known as heartworm. This filarioid is transmitted by culicid vectors and primarily affects dogs, but other animals may also become infected, such as wild ...Dirofilaria immitis is an important nematode parasite commonly known as heartworm. This filarioid is transmitted by culicid vectors and primarily affects dogs, but other animals may also become infected, such as wild carnivores, cats and humans. The aim of the present study was to assess the development of D. immitis larvae in different culicid populations under laboratory conditions. Adult females of populations of Aedes aegypti from the city of Recife (P1), the city of Campinas (P2) and the Rockefeller strain from the Centers for Disease Control (P3) and one population of Aedes albopictus from Recife (P4) were fed for two hours with infected dog blood containing 2,000 microfilariae/ml of D. immitis. After artificial feeding, the specimens were maintained under controlled conditions. Ten females from each population were dissected daily over 14 days. The infection ratio and vector efficiency index were calculated and D. immitis development from L1 to L3 was assessed. The larvae in P1, P2, P3 and P4 reached the third stage in 11, 10, 14 and 9 days, respectively. The vector efficiency index was 53.8%, 20.0%, 7.4% and 25.2% in P1, P2, P3 and P4, respectively. The findings demonstrate that D. immitis larvae develop in all culicid populations studied herein. Based on mosquito mortality, development time and VEI the A. albopictus population from Recife (P4) demonstrated the best performance as vector. This is the second report of D. immitis development in A. albopictus from Brazil. The present data reinforce the role of this species as vector of D. immitis in an area where greater importance has long been given to A. aegypti.展开更多
This study aimed to evaluate the occurrence of EIPH in vaquejada horses via endoscopy and tracheal lavage and citology. We used eight Quarter-mile horses that usually perform at vaquejadas. Clinical examination was ca...This study aimed to evaluate the occurrence of EIPH in vaquejada horses via endoscopy and tracheal lavage and citology. We used eight Quarter-mile horses that usually perform at vaquejadas. Clinical examination was carried out before the race. After the race, endoscopy and tracheal lavage with 20 ml of saline via the service channel of the endoscope were performed. The samples were sent to the laboratory for processing and confection of the slides. Of the eight horses (four pull and four helper horses), six had hemosiderophages in their tracheal aspirates, confirming the existence of pulmonary hemorrhage after exercise. No difference was observed between the analyzed parameters between the two groups of vaquejada horses (P > 0.05). Tracheal lavage and endoscopy can be used for evaluating this important disease in animal athletes.展开更多
The biosurfactant produced by Candida sphaerica in a fermentor containing 5% vegetal oil refinery waste and 2.5% was tested in the removal of motor oil from soils and seawater. In kinetic assays, the isolated biosurfa...The biosurfactant produced by Candida sphaerica in a fermentor containing 5% vegetal oil refinery waste and 2.5% was tested in the removal of motor oil from soils and seawater. In kinetic assays, the isolated biosurfactant removed more than 86% of the motor oil adsorbed to clay, silty and sandy soils at the critical micelle concentration （CMC）. Static removal tests performed in glass columns demonstrated that the crude biosurfactant was able to remove 75% and 92% of the oil contained in clay and silty soil, respectively, whereas the isolated biosurfactant at its CMC removed 50% of the oil from sandy soil. In the washing of hydrophobic compound on a porous surface, the removal rate was 60%. The biosurfactant also proved to be efficient in detergency tests since the crude surfactant removed 41% of motor oil from contaminated cotton cloth. In tests carried out with seawater, the crude biosurfactant showed an oil spreading efficiency of 75% in both screening dispersion test and oil displacement efficiency methods. Regarding the swirling bottle test, the dispersion rate was 72% for the isolated biosurfactant at a concentration twice the CMC. The biosurfactant studied demonstrated potential for application as an adjuvant in biotechnological processes for environmental decontamination.展开更多
This study aimed at evaluating the quality of melon Pele de Sapo “Mabel” and Yellow melon “UFERSA-05” minimally processed preserved in different packages. The fruits were harvested at Mossoró-RN and transport...This study aimed at evaluating the quality of melon Pele de Sapo “Mabel” and Yellow melon “UFERSA-05” minimally processed preserved in different packages. The fruits were harvested at Mossoró-RN and transported to Serra Talhada-PE. In the first study, melon fruit Piel de Sapo “Mabel” was selected, washed, weighed, cooled, peeled, cut into cube shape, sanitized in chlorine solution, drained, packaged in rigid polypropylene tray, sealed with polypropylene film or polypropylene rigid cap and kept for 8 days at 8℃. In the second study, fruits of yellow melon “UFERSA-05” and Pele de Sapo “Mabel” were minimally processed and kept in rigid polypropylene tray, sealed with polypropylene film and stored for 8 days at 8℃. There was no significant interaction between packaging (sealed with film and polypropylene cover) and period of conservation, for pH, total titratable acidity (TTA), total soluble solids (TSS) and Loss of fresh mass (LFM) for Melon “Mabel”. While there was significant interaction between types of melon (“UFERSA-05” and “Mabel”) and storage period (0, 2, 4, 6, and 8 days) for pH, TTA and TSS. In sensory evaluation were noticeable changes in appearance, flavor, aroma and flesh firmness, characterized by translucency, alcoholic aroma and softening in “Mabel” melon kept in tray with lid. Melon “UFERSA-05” showed lower pH, total soluble solids, total soluble sugars, PME activity, lack of translucency high flesh firmness compared to “Mabel” melon during storage. The high levels of sugars in melon “Mabel” may be related to the incidence of translucency, which was not observed in “UFERSA-05” melon, with a strong potential to minimal processing.展开更多
The aim of this investigation was to analyze two samples of pollen from jandaíra stingless bees (Melipona subnitida) in view of their mineral composition, free amino acids and sugars. Palynological analysis showe...The aim of this investigation was to analyze two samples of pollen from jandaíra stingless bees (Melipona subnitida) in view of their mineral composition, free amino acids and sugars. Palynological analysis showed that the predominant pollen was monofloral from Senna sp. species (94.5%, pollen 2011) and the second pollen sample showed the presence of two primary species, Chamaecrista sp. (39.2% pollen 2009) and Mimosa tenuiflora (43.5%) (pollen 2009). The highest mineral content was potassium. The bee pollen contained 20.8% and 31.0% of mannitol in samples from 2011 and 2009, respectively. Proline and serine are the predominant amino acids. High content of essential amino acids, minerals and the sugar mannitol confirmed high nutritional value of pollen samples from jandaíra bees.展开更多
Brazil is responsible for 27% of the world production of soybeans and 7% of maize. Mato Grosso and Para states in Brazil are among the largest producer. The viability to the cultivation of maize (Zea mays) and soybean...Brazil is responsible for 27% of the world production of soybeans and 7% of maize. Mato Grosso and Para states in Brazil are among the largest producer. The viability to the cultivation of maize (Zea mays) and soybeans (Glycine max), for future climate scenarios (2070-2100, GHG) is evaluated based on crop modeling (DSSAT) forced by observational data and regional climate simulations (HadRM3). The results demonstrated that a substantial reduction in the yield in particular for maize may be expected for the end of the 21st century. Distinct results are found for soybeans. By applying the A2 climate changes scenario, soybean yield rises by up top 60% assuming optimum soil treatment and no water stress. However, by analyzing the inter-annual variability of crop yields for both maize and soybean, could be demonstrated larger year-to-year fluctuations under greenhouse warming conditions as compared to current conditions, leading to very low productivity by the end of the 21st century. Therefore, these Brazilian states do not appear to be economically suitable for a future cultivation of maize and soybeans. Improved adaptation measures and soil management may however partially alleviate the negative climate change effect.展开更多
The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, i...The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, indicating the great physical effort during competitions and with that could impact welfare. To test the hypothesis that vaquejada horses can be evaluated for the quality of welfare through a combination of non-invasive and invasive methods, an experiment was developed that aimed to assess the quality of welfare through thermography and blood biomarkers before and after vaquejada races. Ten vaquejada horses, which were in regular competition, were submitted to the vaquejadas racing test were used. Thermography was performed in 14 regions of interest (head, neck and thorax) in the following phases: pre-race and +1, +4 and +24 hours of the races. Blood samples were collected in pre-race, immediately after and +1, +4 and +24 hours of the races. Results were submitted to ANOVA and Tukey’s test, with p set at 5%. Thermography showed that local temperatures before the race and +24 hours after the races were similar (p > 0.05), as well as between +1 and +4 hours after the races (p > 0.05), which showed higher surface temperatures than the first group (pre-race and +24 hours) (p 0.05) after the exercises. In conclusion, the vaquejada horses evaluated by infrared thermography and blood analyses maintained the quality of well-being, with the physiological elevation of several parameters after the races and recovery in less than 24 hours after the races. Thermographic images were also able to help in the analysis of the regions of interest that have contact with harnesses, demonstrating that the use of adequate and correct equipment does not compromise animal welfare.展开更多
In recent decades, the intensity of training and equestrian competitions has significantly increased, thus the assessment of the well-being of the equine athlete has become essential in all equestrian modalities. The ...In recent decades, the intensity of training and equestrian competitions has significantly increased, thus the assessment of the well-being of the equine athlete has become essential in all equestrian modalities. The aim of this study was to ascertain whether equine athletes submitted to a vaquejada simulation test (VqST), comprised of three races, presented changes in blood biomarkers related to stress and health status. Fourteen healthy Quarter Horses, used as pull horses in this equestrian modality, were evaluated. Ten animals were submitted to the VqST and the remaining four were used as a control group. Blood samples were collected pre-test (during fast), immediately after, and at 1, 4 and 24 hours of recovery. The assessed blood biomarkers included cortisol, interleukin (IL)-6, IL-1β, iron, urea, creatinine, and gamma-glutamyl transferase (GGT) concentrations and results were analyzed using One Way ANOVA (time) with the SigmaStat 13.0 software. No differences between sample times were detected in both groups (p > 0.05) and no differences were found between groups (p > 0.05). The results suggest that all horses were well conditioned for the level of effort imposed by the three vaquejada races. The adaptation to physical exercise may enable the regulation of the acute response to stress in the tissues involved in the exercises, with no differences being observed in stress and health biomarkers, such as IL-6, IL-1β and cortisol. In conclusion, well-conditioned vaquejada horses exhibit a balanced regulation of biological processes, which contributes an increased athletic longevity and better quality of athletic life.展开更多
OBJECTIVE To analyze the whole transcriptome of zoanthid Protopalythoa variabilis(P.variabilis),a cnidarian,and discover the potential toxic substances in P.variabilis.METHODS The P.variabilis RNA deep sequencing was ...OBJECTIVE To analyze the whole transcriptome of zoanthid Protopalythoa variabilis(P.variabilis),a cnidarian,and discover the potential toxic substances in P.variabilis.METHODS The P.variabilis RNA deep sequencing was performed using the HiSeq 2500 automatic sequencing platform.All the unigenes generated from the assembly process were functionally annotated based on the similarity with databases.The multiple alignments of translated toxin-related sequences were performed with Clustalw2,and amino acid identity and similarity highlighted by using BoxShade tool.Three different methods including ITASSER,PEP-FOLD and MODELLER were applied to predict tri-dimensional models of toxin-related polypeptides from translated transcript sequences of P.variabilis.The toxicity of one of the putative toxins,namely ShK/Aurelin-like peptide,was evaluated using zebrafish model.RESULTS A total of 67,549,914 pairs of quality-filtered,90-base-pair Illumina reads from an mRNA sample were obtained.The de novo assemblies yielded 276,526 contigs.The sequence comparison of 130,121 unigenes with entries in Toxin database showed that 1542 unigenes were potential peptide toxins at which 11 unigenes were related to Stichodactyla toxin(ShK)domain(Pfam ID:PF01549).ShK is a 35 residues peptide sequence that was firstly discovered from the sea anemone Stichodactyla helianthus.Here,we found out one ShK-like peptide that processed a relatively higher sequence similarity with known ShK(Uniprot ID:P29186)of Bunodosoma granuliferum(red warty sea anemone).The Protopalythoa Shk-like peptide was submitted to Probis server to detect probable binding site and found to match with a protein AURELIN(PDB id:2lg4,UniProt id:Q0MWV8)which possesses structural homology with previously identified antimicrobial peptides and K+-channel-blocking toxins.Our results showed that the ShK/Aurelin-like peptide was lethal to zebrafish embryos at concentrations above 30-μmol·L1,and could induce zebrafish locomotor deficit at 10μmol·L-1.CONCLUSION This study,for the first time,presented the whole transcriptome profile and a potential toxic peptide of P.variabilis.展开更多
This study describes the phytochemical profile and antioxidant activity of an extract from the leaves of Pouteria caimito (Sapotaceae). The extract in ethanol was obtained by maceration at room temperature and subject...This study describes the phytochemical profile and antioxidant activity of an extract from the leaves of Pouteria caimito (Sapotaceae). The extract in ethanol was obtained by maceration at room temperature and subjected to the liquid-liquid partition to obtain fractions in hexane and ethyl acetate. Steroids, triterpenes, saponins, alkaloids and flavonoids were identified by the phytochemical prospection of extracts and fractions from the leaves. The analysis of the ethyl acetate fraction by UPLC-QTOF-MS allowed us to identify eight triterpenes, namely, euscaphic acid (1), hyptadienic acid (2), betulinic acid (3), oleanolic acid (4), ursolic acid (5), 3β-(O-p-coumaroyl)- alphitolic acid (6), 3β-(O-p-coumaroyl)-maslinic acid (7) and 3β-(O-p-coumaroyl)-2-hydroxy-urs-12-en-28-oic acid (8). The ethanol extract and ethyl acetate fraction presented total phenolic contents of 10.6 ± 0.1 and 11.4 ± 0.3 mg GAE g-1, respectively, and considerable antioxidant activity in the DPPH assay with EC50 values of 299.4 ± 1.5 and 391.8 ± 0.9 μg·ml-1, respectively.展开更多
Intestinal nematodes are among the most common parasites of dogs, being Toxocara spp. and Ancylostoma spp. threats to public health due to their zoonotic potential. In this study, three parasitological diagnostic tech...Intestinal nematodes are among the most common parasites of dogs, being Toxocara spp. and Ancylostoma spp. threats to public health due to their zoonotic potential. In this study, three parasitological diagnostic techniques for detecting the eggs/oocysts of canine intestinal parasites were evaluated. Fecal samples (n = 285) were collected from the environment (i.e. public streets, squares and homes) in Garanhuns, Pernambuco, Brazil. Samples were analyzed using the Willis-Mollay simple flotation technique, spontaneous sedimentation (Hoffman, Pons and Janer) and modified centrifugal flotation. Among the samples analyzed, 56.49% (161/285) showed eggs and/or oocysts of intestinal parasites. In particular, 44.21% (126/285) were detected by the Willis technique, 45.14% (121/268) by centrifugal flotation and 31.57% (90/285) by the Hoffmann technique. The kappa index indicated good concordance between the centrifugal flotation and Willis techniques (κ = 0.625), whereas fair concordance was observed between Hoffmann and Willis (κ = 0.480), and between Hoffman and centrifugal flotation (κ = 0.433). In conclusion, the centrifugal flotation technique showed high sensitivity towards detecting Ancylostoma spp. and Toxocara spp. (the most common parasites in this study) and could be used in association with the Willis technique to obtain safe diagnoses within routine laboratory analysis.展开更多
Our objectives were to measure glutamine (Gln) and glutamate (Glu) concentrations in the milk and blood of lactating Holstein cows, as well as milk composition and somatic cell count (SCC) throughout different stages ...Our objectives were to measure glutamine (Gln) and glutamate (Glu) concentrations in the milk and blood of lactating Holstein cows, as well as milk composition and somatic cell count (SCC) throughout different stages of lactation. Samples were collected from 21 cows in the 1st, 2nd, 3rd, 5th and 10th months of lactation. It analyzed Gln and Glu in the blood and Gln, Glu, lactose, fat, protein and somatic cells in the milk. Also, it measured Gln and Glu in blood from these dairy cows’ calves. Results were analyzed using one-way ANOVA, T-test and Tukey’s test, with a 5% significance level. There was a difference in blood [Gln] in from dairy cows (P nd (~0.181 μmol/mL) and 5th (~0.190 μmol/mL) months of lactation and the highest at 10th month (~0.276 μmol/mL). Also there was a higher protein content in the 5th and 10th months of lactation (~3.61% each) when compared to the other months (P < 0.05). There was no difference in calves’ parameters and in all others parameters from dairy cows. We concluded that the lactation period produced a significant interference in blood [Gln] but not in milk [Gln] which may indicate increase in utilization or decrease in production of this amino acids during lactation in these cows under tropical conditions.展开更多
Endophytic bacteria may influence agricultural production in several ways, including promoting plant growth. Two experiments were conducted in order to evaluate the combination of endophytic bacteria from the Brazilia...Endophytic bacteria may influence agricultural production in several ways, including promoting plant growth. Two experiments were conducted in order to evaluate the combination of endophytic bacteria from the Brazilian Northeast region aims at the commercial introduction of the inoculation of these bacteria in micropropagated sugarcane plants using a temporary immersion bioreactor. One experiment was done in tubes with sterile commercial substrate, and the other was done in pots with soil;both were installed in a greenhouse. A mixed inoculation was performed in six inoculated endophytic diazotrophic bacteria in micropropagated sugarcane plants, variety RB92579. In the experiment with soil, the mixed inoculation significantly increased the shoot dry matter of plants without the addition of nitrogen fertilizer. However, the accumulation of total-N in the tissues showed no significant differences between treatments with and without nitrogen fertilization. The evaluation of micropropagated seedlings showed no increases in the parameters tested. The results showed that the response of inoculation in temporary immersion bioreactor micropropagation is possible, and that the application of homologous strains may have contributed to a better response by the interaction of endophytic bacteria with sugarcane RB92579. Further studies should be conducted to improve the methodology, which indicates a great potential to optimize this process on a commercial scale.展开更多
<span style="font-family:Verdana;">Chimaeras</span><span style="font-family:;" "=""><span style="font-family:Verdana;">, fish in the order Chimaerif...<span style="font-family:Verdana;">Chimaeras</span><span style="font-family:;" "=""><span style="font-family:Verdana;">, fish in the order Chimaeriformes, are among the rarest and least studied fish in the class Chondrichthyes (cartilaginous fish). Previous records have indicated that a species of chimaera known as the elephantfish, or cockfish, </span><i><span style="font-family:Verdana;">Callorhinchus callorynchus</span></i><span style="font-family:Verdana;">, may be found in marine waters off southeastern Brazil, as far north as the state of S<span style="font-size:10.0pt;font-family:"">ã</span>o Paulo. Here we report that </span><i><span style="font-family:Verdana;">C. callorynchus</span></i><span style="font-family:Verdana;">, caught with bottom gillnet, was found in an urban fish market in the city of Rio de Janeiro (~23<span style="white-space:nowrap;">˚</span>S). This and other records from a variety of places call into question the distribution limits of the American elephantfish in the southwestern Atlantic Ocean and whether, at least seasonally, this species may often be found as far north as the state of Rio de Janeiro. Because of scientific and conservation interests in the poorly known Chimaeriformes, we recommend long-term monitoring of the fish catch in ports along the southern Brazilian coast, to better understand the natural history of the intriguing chimeras.</span></span>展开更多
The aim of the present study was to test the hypothesis that glutathione peroxidase, uric acid, and a number of hematological biomarkers do not vary in adult mares, over a 24-hour period. Seven adult (age 10 ± 6 ...The aim of the present study was to test the hypothesis that glutathione peroxidase, uric acid, and a number of hematological biomarkers do not vary in adult mares, over a 24-hour period. Seven adult (age 10 ± 6 yrs;weight 370 ± 30 kg) Arabian Purebred mares were used. Blood samples were collected every two hours, except during the period after-meals, when samples were collected every 30 minutes, totaling four samples in two hours. These samples were used to analyze glutathione peroxidase (GPx), uric acid (UA), glucose (GLU), total plasma protein (TPP), red blood cells (RBC), hemoglobin (HB), hematocrit (HT), red cell distribution width (RDW), white blood cells (WBC) and lymphocytes (LYM). One-way repeated measures analysis of variance (ANOVA) was used to determine significant differences. Tukey’s test was used for multiple comparisons between the averages. Ρ展开更多
The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and l...The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and longevity of their females. The objective of this research was to evaluate the development and reproduction of the predator Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae) fed with Tenebrio molitor L., 1785 (Coleoptera: Tenebrionidae) pupae in a Eucalyptus grandis Hill ex Maiden (Myrtaceae) plant in the field. The duration and survival of the nymphal stage of B. tabidus were 32.07 ± 8.95 days and 77.00 ± 0.42%, respectively with 15.55 ± 8.19 egg masses, 603.00 ± 379.20 eggs and 387.45 ± 291.40 nymphs per female of this predator while longevity of its males and females was 119.80 ± 32.80 and 160.20 ± 42.13 days, respectively. Better results with E. grandis plant in the field and the fact that B. tabidus will be adapted at release time show that this predator should be reared in these conditions.展开更多
基金Supported by the Oswaldo Cruz Foundation(FIOCRUZ)the Pernambuco Science and Technology Support Foundation(FACEPE)(PROEP-FIOCRUZ 19/2015)+2 种基金the National Council of Technological and Scientific Development(CNPq)(Processes APQ 0906-2.11/08)the National Council for the Improvement of Higher Education(CAPES)the Intramural Research Program of the National Institutes of Health(LPD/NIAID/NIH)
文摘To evaluate the therapeutic effects of bone marrow-derived CD11b<sup>+</sup>CD14<sup>+</sup> monocytes in a murine model of chronic liver damage.METHODSChronic liver damage was induced in C57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent assay.RESULTSCD11b<sup>+</sup>CD14<sup>+</sup> monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. CD11b<sup>+</sup>CD14<sup>+</sup> monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and osteopontin.CONCLUSIONMonocyte therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.
文摘Several studies have shown a major development in height and cannon bone circumference in foals from birth to weaning and from different breeds. However, in Brazil, there is little information about foal development. For this reason, this study has used data on foals from seven different farms in Pernambuco, Northeast Brazil, where horses have a major economic importance. The aim of this study was to compare foal’s development from four breeds and to develop a regression formula in order to estimate such development. It was used data from 210 suckling foals (Arabians n = 18, Campolina n = 86, Mangalarga Marchador n = 42 and Quarter Horses n = 64), raised in similar management practices. Their height at withers, heart girth, and cannon bone girth were measured monthly from birth to weaning. Data we reanalyzed by ANOVA and Tukey Test, with P established in 5%. The highest mean of height at withers was observed in the Arabian foals, and it was similar to Campolina and Mangalarga Marchador foals. The last breed presented a higher percentage of gain in height (~36%) and higher heart girth. On the other hand, Campolina foals had the highest mean of cannon bone girth. There was a significant interaction between breed and development phase in heart and cannon bone girths. All regression for mulas obtained from this data was very significant, and it was showed that they can predict height at withers in the four breeds studied. Heart and cannon bone girths showed medium value of R2 and large significance. It is important to highlight that fetal programing produces significant impact on foal development, even after weaning. So, this study would have observed some more changes if its scope had been extended beyond this phase.
文摘The monitoring and development of foals, based on biometric indices, and the determination of body composition could be important for genetic improvement and the elaboration of nutritional plans for horses. Considering that little information was available, the aim of the present study was to assess the growth and determine the body composition of suckling Quarter Horse foals. Animals (n = 46) of both sexes were monitored monthly from birth until the fifth month of age, using an ultrasound device and the following measurements: Wither Height (WH);Chest Perimeter (CP);Cannon Bone Circumference (CBC) Cannon Bone Girth (CG);Body Mass (BM);and Rump Fat Thickness (RFT). These tests determined the Fat-Free Mass (FFM), BM, Fat Percentage (FP) and Fat Thickness (FT). The data were analyzed using ANOVA and Tukey’s test, with differences considered statistically significant when P ≤ 0.05. The results demonstrated that the parameters analyzed changed significantly during the study period. The BM was ~41 at birth and ~177 kg at the point of weaning. Conversely, the FM was ~3.8 at birth and 18.1 kg at the point of weaning. The FFM was ~37 and ~160 kg at the time of birth and weaning, respectively. The FP varied from 9.3 at birth to 9.54 at weaning. The mean FT was ~1.39 and ~1.91 cm at birth and weaning, respectively. The WH measured ~91 at birth and 116 cm at weaning. In conclusion, suckling foals gain BM quickly from the time of birth to the time of weaning. The main cause of this gain is FFM. Therefore, due to the significant changes in the parameters studied herein, it is important to monitor these values in order to prevent nutritional imbalances.
文摘Dirofilaria immitis is an important nematode parasite commonly known as heartworm. This filarioid is transmitted by culicid vectors and primarily affects dogs, but other animals may also become infected, such as wild carnivores, cats and humans. The aim of the present study was to assess the development of D. immitis larvae in different culicid populations under laboratory conditions. Adult females of populations of Aedes aegypti from the city of Recife (P1), the city of Campinas (P2) and the Rockefeller strain from the Centers for Disease Control (P3) and one population of Aedes albopictus from Recife (P4) were fed for two hours with infected dog blood containing 2,000 microfilariae/ml of D. immitis. After artificial feeding, the specimens were maintained under controlled conditions. Ten females from each population were dissected daily over 14 days. The infection ratio and vector efficiency index were calculated and D. immitis development from L1 to L3 was assessed. The larvae in P1, P2, P3 and P4 reached the third stage in 11, 10, 14 and 9 days, respectively. The vector efficiency index was 53.8%, 20.0%, 7.4% and 25.2% in P1, P2, P3 and P4, respectively. The findings demonstrate that D. immitis larvae develop in all culicid populations studied herein. Based on mosquito mortality, development time and VEI the A. albopictus population from Recife (P4) demonstrated the best performance as vector. This is the second report of D. immitis development in A. albopictus from Brazil. The present data reinforce the role of this species as vector of D. immitis in an area where greater importance has long been given to A. aegypti.
文摘This study aimed to evaluate the occurrence of EIPH in vaquejada horses via endoscopy and tracheal lavage and citology. We used eight Quarter-mile horses that usually perform at vaquejadas. Clinical examination was carried out before the race. After the race, endoscopy and tracheal lavage with 20 ml of saline via the service channel of the endoscope were performed. The samples were sent to the laboratory for processing and confection of the slides. Of the eight horses (four pull and four helper horses), six had hemosiderophages in their tracheal aspirates, confirming the existence of pulmonary hemorrhage after exercise. No difference was observed between the analyzed parameters between the two groups of vaquejada horses (P > 0.05). Tracheal lavage and endoscopy can be used for evaluating this important disease in animal athletes.
文摘The biosurfactant produced by Candida sphaerica in a fermentor containing 5% vegetal oil refinery waste and 2.5% was tested in the removal of motor oil from soils and seawater. In kinetic assays, the isolated biosurfactant removed more than 86% of the motor oil adsorbed to clay, silty and sandy soils at the critical micelle concentration （CMC）. Static removal tests performed in glass columns demonstrated that the crude biosurfactant was able to remove 75% and 92% of the oil contained in clay and silty soil, respectively, whereas the isolated biosurfactant at its CMC removed 50% of the oil from sandy soil. In the washing of hydrophobic compound on a porous surface, the removal rate was 60%. The biosurfactant also proved to be efficient in detergency tests since the crude surfactant removed 41% of motor oil from contaminated cotton cloth. In tests carried out with seawater, the crude biosurfactant showed an oil spreading efficiency of 75% in both screening dispersion test and oil displacement efficiency methods. Regarding the swirling bottle test, the dispersion rate was 72% for the isolated biosurfactant at a concentration twice the CMC. The biosurfactant studied demonstrated potential for application as an adjuvant in biotechnological processes for environmental decontamination.
基金The authors thank the Universidade Federal Rural do Pernambuco,the Universidade Federal Rural do Semiárido,CNPq,FACEPE and CAPES for the financial support.
文摘This study aimed at evaluating the quality of melon Pele de Sapo “Mabel” and Yellow melon “UFERSA-05” minimally processed preserved in different packages. The fruits were harvested at Mossoró-RN and transported to Serra Talhada-PE. In the first study, melon fruit Piel de Sapo “Mabel” was selected, washed, weighed, cooled, peeled, cut into cube shape, sanitized in chlorine solution, drained, packaged in rigid polypropylene tray, sealed with polypropylene film or polypropylene rigid cap and kept for 8 days at 8℃. In the second study, fruits of yellow melon “UFERSA-05” and Pele de Sapo “Mabel” were minimally processed and kept in rigid polypropylene tray, sealed with polypropylene film and stored for 8 days at 8℃. There was no significant interaction between packaging (sealed with film and polypropylene cover) and period of conservation, for pH, total titratable acidity (TTA), total soluble solids (TSS) and Loss of fresh mass (LFM) for Melon “Mabel”. While there was significant interaction between types of melon (“UFERSA-05” and “Mabel”) and storage period (0, 2, 4, 6, and 8 days) for pH, TTA and TSS. In sensory evaluation were noticeable changes in appearance, flavor, aroma and flesh firmness, characterized by translucency, alcoholic aroma and softening in “Mabel” melon kept in tray with lid. Melon “UFERSA-05” showed lower pH, total soluble solids, total soluble sugars, PME activity, lack of translucency high flesh firmness compared to “Mabel” melon during storage. The high levels of sugars in melon “Mabel” may be related to the incidence of translucency, which was not observed in “UFERSA-05” melon, with a strong potential to minimal processing.
基金supported by grants from FACEPE(Grant no.PRONEM APQ-1232.1.06/10),CNPq-PPBio 503285/2009-9)and CAPES.
文摘The aim of this investigation was to analyze two samples of pollen from jandaíra stingless bees (Melipona subnitida) in view of their mineral composition, free amino acids and sugars. Palynological analysis showed that the predominant pollen was monofloral from Senna sp. species (94.5%, pollen 2011) and the second pollen sample showed the presence of two primary species, Chamaecrista sp. (39.2% pollen 2009) and Mimosa tenuiflora (43.5%) (pollen 2009). The highest mineral content was potassium. The bee pollen contained 20.8% and 31.0% of mannitol in samples from 2011 and 2009, respectively. Proline and serine are the predominant amino acids. High content of essential amino acids, minerals and the sugar mannitol confirmed high nutritional value of pollen samples from jandaíra bees.
基金This study has been sponsored by the National Institute of Science and Technology Climate Change and by the SoCoCA project funded by the Research Council of Norway(contract 190159)Moreover support has been provided by the sub-rede Agricultura do MCTI and by the INCT-Mudanças Climáticas.
文摘Brazil is responsible for 27% of the world production of soybeans and 7% of maize. Mato Grosso and Para states in Brazil are among the largest producer. The viability to the cultivation of maize (Zea mays) and soybeans (Glycine max), for future climate scenarios (2070-2100, GHG) is evaluated based on crop modeling (DSSAT) forced by observational data and regional climate simulations (HadRM3). The results demonstrated that a substantial reduction in the yield in particular for maize may be expected for the end of the 21st century. Distinct results are found for soybeans. By applying the A2 climate changes scenario, soybean yield rises by up top 60% assuming optimum soil treatment and no water stress. However, by analyzing the inter-annual variability of crop yields for both maize and soybean, could be demonstrated larger year-to-year fluctuations under greenhouse warming conditions as compared to current conditions, leading to very low productivity by the end of the 21st century. Therefore, these Brazilian states do not appear to be economically suitable for a future cultivation of maize and soybeans. Improved adaptation measures and soil management may however partially alleviate the negative climate change effect.
文摘The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, indicating the great physical effort during competitions and with that could impact welfare. To test the hypothesis that vaquejada horses can be evaluated for the quality of welfare through a combination of non-invasive and invasive methods, an experiment was developed that aimed to assess the quality of welfare through thermography and blood biomarkers before and after vaquejada races. Ten vaquejada horses, which were in regular competition, were submitted to the vaquejadas racing test were used. Thermography was performed in 14 regions of interest (head, neck and thorax) in the following phases: pre-race and +1, +4 and +24 hours of the races. Blood samples were collected in pre-race, immediately after and +1, +4 and +24 hours of the races. Results were submitted to ANOVA and Tukey’s test, with p set at 5%. Thermography showed that local temperatures before the race and +24 hours after the races were similar (p > 0.05), as well as between +1 and +4 hours after the races (p > 0.05), which showed higher surface temperatures than the first group (pre-race and +24 hours) (p 0.05) after the exercises. In conclusion, the vaquejada horses evaluated by infrared thermography and blood analyses maintained the quality of well-being, with the physiological elevation of several parameters after the races and recovery in less than 24 hours after the races. Thermographic images were also able to help in the analysis of the regions of interest that have contact with harnesses, demonstrating that the use of adequate and correct equipment does not compromise animal welfare.
文摘In recent decades, the intensity of training and equestrian competitions has significantly increased, thus the assessment of the well-being of the equine athlete has become essential in all equestrian modalities. The aim of this study was to ascertain whether equine athletes submitted to a vaquejada simulation test (VqST), comprised of three races, presented changes in blood biomarkers related to stress and health status. Fourteen healthy Quarter Horses, used as pull horses in this equestrian modality, were evaluated. Ten animals were submitted to the VqST and the remaining four were used as a control group. Blood samples were collected pre-test (during fast), immediately after, and at 1, 4 and 24 hours of recovery. The assessed blood biomarkers included cortisol, interleukin (IL)-6, IL-1β, iron, urea, creatinine, and gamma-glutamyl transferase (GGT) concentrations and results were analyzed using One Way ANOVA (time) with the SigmaStat 13.0 software. No differences between sample times were detected in both groups (p > 0.05) and no differences were found between groups (p > 0.05). The results suggest that all horses were well conditioned for the level of effort imposed by the three vaquejada races. The adaptation to physical exercise may enable the regulation of the acute response to stress in the tissues involved in the exercises, with no differences being observed in stress and health biomarkers, such as IL-6, IL-1β and cortisol. In conclusion, well-conditioned vaquejada horses exhibit a balanced regulation of biological processes, which contributes an increased athletic longevity and better quality of athletic life.
基金The project supported by grants from the Science and Technology Development Fund of Macao,China(058/2009and 078/2011/A3)Research Committee,University of Macao〔MYRG138(Y1-Y4)-ICMS12-LMY and MYRG139(Y1-Y4)-ICMS-LMY〕
文摘OBJECTIVE To analyze the whole transcriptome of zoanthid Protopalythoa variabilis(P.variabilis),a cnidarian,and discover the potential toxic substances in P.variabilis.METHODS The P.variabilis RNA deep sequencing was performed using the HiSeq 2500 automatic sequencing platform.All the unigenes generated from the assembly process were functionally annotated based on the similarity with databases.The multiple alignments of translated toxin-related sequences were performed with Clustalw2,and amino acid identity and similarity highlighted by using BoxShade tool.Three different methods including ITASSER,PEP-FOLD and MODELLER were applied to predict tri-dimensional models of toxin-related polypeptides from translated transcript sequences of P.variabilis.The toxicity of one of the putative toxins,namely ShK/Aurelin-like peptide,was evaluated using zebrafish model.RESULTS A total of 67,549,914 pairs of quality-filtered,90-base-pair Illumina reads from an mRNA sample were obtained.The de novo assemblies yielded 276,526 contigs.The sequence comparison of 130,121 unigenes with entries in Toxin database showed that 1542 unigenes were potential peptide toxins at which 11 unigenes were related to Stichodactyla toxin(ShK)domain(Pfam ID:PF01549).ShK is a 35 residues peptide sequence that was firstly discovered from the sea anemone Stichodactyla helianthus.Here,we found out one ShK-like peptide that processed a relatively higher sequence similarity with known ShK(Uniprot ID:P29186)of Bunodosoma granuliferum(red warty sea anemone).The Protopalythoa Shk-like peptide was submitted to Probis server to detect probable binding site and found to match with a protein AURELIN(PDB id:2lg4,UniProt id:Q0MWV8)which possesses structural homology with previously identified antimicrobial peptides and K+-channel-blocking toxins.Our results showed that the ShK/Aurelin-like peptide was lethal to zebrafish embryos at concentrations above 30-μmol·L1,and could induce zebrafish locomotor deficit at 10μmol·L-1.CONCLUSION This study,for the first time,presented the whole transcriptome profile and a potential toxic peptide of P.variabilis.
文摘This study describes the phytochemical profile and antioxidant activity of an extract from the leaves of Pouteria caimito (Sapotaceae). The extract in ethanol was obtained by maceration at room temperature and subjected to the liquid-liquid partition to obtain fractions in hexane and ethyl acetate. Steroids, triterpenes, saponins, alkaloids and flavonoids were identified by the phytochemical prospection of extracts and fractions from the leaves. The analysis of the ethyl acetate fraction by UPLC-QTOF-MS allowed us to identify eight triterpenes, namely, euscaphic acid (1), hyptadienic acid (2), betulinic acid (3), oleanolic acid (4), ursolic acid (5), 3β-(O-p-coumaroyl)- alphitolic acid (6), 3β-(O-p-coumaroyl)-maslinic acid (7) and 3β-(O-p-coumaroyl)-2-hydroxy-urs-12-en-28-oic acid (8). The ethanol extract and ethyl acetate fraction presented total phenolic contents of 10.6 ± 0.1 and 11.4 ± 0.3 mg GAE g-1, respectively, and considerable antioxidant activity in the DPPH assay with EC50 values of 299.4 ± 1.5 and 391.8 ± 0.9 μg·ml-1, respectively.
文摘Intestinal nematodes are among the most common parasites of dogs, being Toxocara spp. and Ancylostoma spp. threats to public health due to their zoonotic potential. In this study, three parasitological diagnostic techniques for detecting the eggs/oocysts of canine intestinal parasites were evaluated. Fecal samples (n = 285) were collected from the environment (i.e. public streets, squares and homes) in Garanhuns, Pernambuco, Brazil. Samples were analyzed using the Willis-Mollay simple flotation technique, spontaneous sedimentation (Hoffman, Pons and Janer) and modified centrifugal flotation. Among the samples analyzed, 56.49% (161/285) showed eggs and/or oocysts of intestinal parasites. In particular, 44.21% (126/285) were detected by the Willis technique, 45.14% (121/268) by centrifugal flotation and 31.57% (90/285) by the Hoffmann technique. The kappa index indicated good concordance between the centrifugal flotation and Willis techniques (κ = 0.625), whereas fair concordance was observed between Hoffmann and Willis (κ = 0.480), and between Hoffman and centrifugal flotation (κ = 0.433). In conclusion, the centrifugal flotation technique showed high sensitivity towards detecting Ancylostoma spp. and Toxocara spp. (the most common parasites in this study) and could be used in association with the Willis technique to obtain safe diagnoses within routine laboratory analysis.
文摘Our objectives were to measure glutamine (Gln) and glutamate (Glu) concentrations in the milk and blood of lactating Holstein cows, as well as milk composition and somatic cell count (SCC) throughout different stages of lactation. Samples were collected from 21 cows in the 1st, 2nd, 3rd, 5th and 10th months of lactation. It analyzed Gln and Glu in the blood and Gln, Glu, lactose, fat, protein and somatic cells in the milk. Also, it measured Gln and Glu in blood from these dairy cows’ calves. Results were analyzed using one-way ANOVA, T-test and Tukey’s test, with a 5% significance level. There was a difference in blood [Gln] in from dairy cows (P nd (~0.181 μmol/mL) and 5th (~0.190 μmol/mL) months of lactation and the highest at 10th month (~0.276 μmol/mL). Also there was a higher protein content in the 5th and 10th months of lactation (~3.61% each) when compared to the other months (P < 0.05). There was no difference in calves’ parameters and in all others parameters from dairy cows. We concluded that the lactation period produced a significant interference in blood [Gln] but not in milk [Gln] which may indicate increase in utilization or decrease in production of this amino acids during lactation in these cows under tropical conditions.
基金Funding:This work was supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq grant number 310030/2015-3)and MCSB obtained a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES).
文摘Endophytic bacteria may influence agricultural production in several ways, including promoting plant growth. Two experiments were conducted in order to evaluate the combination of endophytic bacteria from the Brazilian Northeast region aims at the commercial introduction of the inoculation of these bacteria in micropropagated sugarcane plants using a temporary immersion bioreactor. One experiment was done in tubes with sterile commercial substrate, and the other was done in pots with soil;both were installed in a greenhouse. A mixed inoculation was performed in six inoculated endophytic diazotrophic bacteria in micropropagated sugarcane plants, variety RB92579. In the experiment with soil, the mixed inoculation significantly increased the shoot dry matter of plants without the addition of nitrogen fertilizer. However, the accumulation of total-N in the tissues showed no significant differences between treatments with and without nitrogen fertilization. The evaluation of micropropagated seedlings showed no increases in the parameters tested. The results showed that the response of inoculation in temporary immersion bioreactor micropropagation is possible, and that the application of homologous strains may have contributed to a better response by the interaction of endophytic bacteria with sugarcane RB92579. Further studies should be conducted to improve the methodology, which indicates a great potential to optimize this process on a commercial scale.
文摘<span style="font-family:Verdana;">Chimaeras</span><span style="font-family:;" "=""><span style="font-family:Verdana;">, fish in the order Chimaeriformes, are among the rarest and least studied fish in the class Chondrichthyes (cartilaginous fish). Previous records have indicated that a species of chimaera known as the elephantfish, or cockfish, </span><i><span style="font-family:Verdana;">Callorhinchus callorynchus</span></i><span style="font-family:Verdana;">, may be found in marine waters off southeastern Brazil, as far north as the state of S<span style="font-size:10.0pt;font-family:"">ã</span>o Paulo. Here we report that </span><i><span style="font-family:Verdana;">C. callorynchus</span></i><span style="font-family:Verdana;">, caught with bottom gillnet, was found in an urban fish market in the city of Rio de Janeiro (~23<span style="white-space:nowrap;">˚</span>S). This and other records from a variety of places call into question the distribution limits of the American elephantfish in the southwestern Atlantic Ocean and whether, at least seasonally, this species may often be found as far north as the state of Rio de Janeiro. Because of scientific and conservation interests in the poorly known Chimaeriformes, we recommend long-term monitoring of the fish catch in ports along the southern Brazilian coast, to better understand the natural history of the intriguing chimeras.</span></span>
文摘The aim of the present study was to test the hypothesis that glutathione peroxidase, uric acid, and a number of hematological biomarkers do not vary in adult mares, over a 24-hour period. Seven adult (age 10 ± 6 yrs;weight 370 ± 30 kg) Arabian Purebred mares were used. Blood samples were collected every two hours, except during the period after-meals, when samples were collected every 30 minutes, totaling four samples in two hours. These samples were used to analyze glutathione peroxidase (GPx), uric acid (UA), glucose (GLU), total plasma protein (TPP), red blood cells (RBC), hemoglobin (HB), hematocrit (HT), red cell distribution width (RDW), white blood cells (WBC) and lymphocytes (LYM). One-way repeated measures analysis of variance (ANOVA) was used to determine significant differences. Tukey’s test was used for multiple comparisons between the averages. Ρ
文摘The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and longevity of their females. The objective of this research was to evaluate the development and reproduction of the predator Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae) fed with Tenebrio molitor L., 1785 (Coleoptera: Tenebrionidae) pupae in a Eucalyptus grandis Hill ex Maiden (Myrtaceae) plant in the field. The duration and survival of the nymphal stage of B. tabidus were 32.07 ± 8.95 days and 77.00 ± 0.42%, respectively with 15.55 ± 8.19 egg masses, 603.00 ± 379.20 eggs and 387.45 ± 291.40 nymphs per female of this predator while longevity of its males and females was 119.80 ± 32.80 and 160.20 ± 42.13 days, respectively. Better results with E. grandis plant in the field and the fact that B. tabidus will be adapted at release time show that this predator should be reared in these conditions.
