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Residual fluoride self-activated effect enabling upgraded utilization of recycled graphite anode
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作者 Shuzhe Yang Qingqing Gao +7 位作者 Yukun Li Hongwei Cai Xiaodan Li Gaoxing Sun Shuxin Zhuang Yujin Tong Hao Luo Mi Lu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第6期24-31,I0002,共9页
Recycling graphite anode from spent lithium-ion batteries(SLIBs)is regarded as a crucial approach to promoting sustainable energy storage industry.However,the recycled graphite(RG)generally presents degraded structure... Recycling graphite anode from spent lithium-ion batteries(SLIBs)is regarded as a crucial approach to promoting sustainable energy storage industry.However,the recycled graphite(RG)generally presents degraded structure and performance.Herein,the residual fluoride self-activated effect is proposed for the upgraded utilization of RG.Simple and low-energy water immersion treatment not only widens the interlayer spacing,but also retains appropriate fluoride on the surface of RG.Theoretical analysis and experiments demonstrate that the residual fluoride can optimize Li~+migration and deposition kinetics,resulting in better Li~+intercalation/deintercalation in the interlayer and more stable Li metal plating/stripping on the surface of RG,As a result,the designed LFP||RG full cells achieve ultrahigh reversibility(~100%Coulombic efficiency),high capacity retention(67%after 200 cycles,0.85 N/P ratio),and commendable adaptability(stable cycling without short-circuiting,0.15 N/P ratio).The energy density is improved from 334 Wh kg^(-1)of 1.1 N/P ratio to 367 Wh kg^(-1)of 0.85 N/P ratio(total mass based on cathode and anode).The exploration of RG by residual fluoride self-activated effect achieves upgraded utilization beyond fresh commercial graphite and highlights a new strategy for efficient reuse of SLIBs. 展开更多
关键词 Spent lithium-ion batteries Recycled graphite anode FLUORIDE Self-activated effect Upgraded utilization
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Clinical and pathophysiological understanding of the hepatorenal syndrome:Still wrong or still not exactly right? 被引量:1
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作者 Benjamin Wilde Ali Canbay Antonios Katsounas 《World Journal of Clinical Cases》 SCIE 2023年第6期1261-1266,共6页
The hepatorenal syndrome(HRS)is one major extrahepatic complication of endstage liver diseases.While circulatory dysregulation is considered as primary etiology for HRS,cirrhosis-related(systemic)inflammation and/or c... The hepatorenal syndrome(HRS)is one major extrahepatic complication of endstage liver diseases.While circulatory dysregulation is considered as primary etiology for HRS,cirrhosis-related(systemic)inflammation and/or cardial dysfunction may also play a key pathogenic role in HRS development.Exclusion of other causes of acute kidney injury(AKI)is required for diagnosis of HRS-AKI by the definition of the International Club of Ascites.However,the pathophysiology of HRS is not understood completely and there are still limited therapeutic options.Reversibility of renal dysfunction after liver transplantation indicates that HRS-AKI is a functional disorder caused by altered cellular function.The interplay between systemic inflammation and the onset of kidneyrelated hypometabolism may have a key role and needs to be studied in depth.This minireview challenges simplified views of the HRS in the context of diagnostics and therapy stressing the need for further evidence to advance the knowledge on this syndrome. 展开更多
关键词 Hepatorenal syndrome Liver disease CIRRHOSIS INFLAMMATION Chronic kidney disease Acute kidney injury
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High-dose biotin neither fosters remyelination nor stimulates malonyl coenzyme A synthesis in the regenerating nerve
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作者 Fabian Szepanowski Rebecca D.Steubing +4 位作者 Cansu Güngör Jaqueline Zipfel Anne K.Mausberg Christoph Kleinschnitz Mark Stettner 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第7期1623-1624,共2页
Dear editor,Dysregulated inflammatory responses primarily account for damage to the central nervous system,however,neurodegenerative processes likely contribute to the pathophysiology of progressive forms of multiple ... Dear editor,Dysregulated inflammatory responses primarily account for damage to the central nervous system,however,neurodegenerative processes likely contribute to the pathophysiology of progressive forms of multiple sclerosis(MS).These pathophysiological aspects include axonal energy depletion and impaired remyelination capacity(Levin et al.,2014).The clinical efficacy of most disease-modifying therapies in MS to date has been achieved by targeting functions of the adaptive immune system(Cree et al.,2019;Szepanowski et al.,2021). 展开更多
关键词 system SYNTHESIS NEITHER
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Combining descriptor-based analyses and mean-field modeling of the electrochemical interface to comprehend trends of catalytic processes at the solid/liquid interface
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作者 Kai S.Exner 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第10期288-290,I0009,共4页
Electrocatalysis is undergoing a renaissance due to its central importance for a sustainable energy economy,relying on green(electro-)chemical processes to harvest,convert,and store energy.Theoretical considerations b... Electrocatalysis is undergoing a renaissance due to its central importance for a sustainable energy economy,relying on green(electro-)chemical processes to harvest,convert,and store energy.Theoretical considerations by electronic structure methods are key to identify potential material motifs for electrocatalytic processes at the solid/liquid interface.Most commonly,heuristic concepts in the realm of materials screening by the compilation of volcano plots are used,which rely on a plethora of simplifications and approximations of the complex electrochemical interface.While the investigation of the catalytic processes at the solid/liquid interface mainly relies on descriptor-based approaches,in the present future article it is discussed that the inclusion of the liquid part of the interface by mean-field models is crucial to elevate screening approaches to the next level. 展开更多
关键词 ELECTROCATALYSIS Volcano plot Descriptor approach Electrochemical interface Mean-field model
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半潜船波浪诱导运动与载荷预报数值方法比较研究
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作者 Qian Gao Changqing Jiang +1 位作者 Youjun Yang Uwe Ritschel 《哈尔滨工程大学学报(英文版)》 CSCD 2023年第3期499-512,共14页
Numerical simulation tools based on potential-flow theory and/or Morison’s equation are widely used for predicting the hydrodynamic responses of floating offshore wind platforms.In general,these simplified approaches... Numerical simulation tools based on potential-flow theory and/or Morison’s equation are widely used for predicting the hydrodynamic responses of floating offshore wind platforms.In general,these simplified approaches are used for the analysis under operational conditions,albeit with a carefully selected approach to account for viscous effects.Nevertheless,due to the limit hydrodynamic modelling to linear and weakly nonlinear models,these approaches severely underpredict the low-frequency nonlinear wave loads and dynamic responses of a semisubmersible.They may not capture important nonlinearities in severe sea states.For the prediction of wave-induced motions and loads on a semisubmersible,this work systematically compares a fully nonlinear viscous-flow solver and a hybrid model combining the potential-flow theory with Morison-drag loads in steep waves.Results show that when nonlinear phenomena are not dominant,the results obtained by the hybrid model and the high-fidelity method show reasonable agreement,while larger discrepancies occur for highly nonlinear regular waves.Specifically,regular waves with various steepness over different frequencies are focused in the present study,which supplements the understanding in applicability of these two groups of method. 展开更多
关键词 Potential flow Viscous flow Moored floating body Steep waves Motions and loads CFD
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Back interface passivation for ultrathin Cu(In,Ga)Se_(2) solar cells with Schottky back contact: A trade-off of electrical effects
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作者 涂野 李勇 殷官超 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第6期621-628,共8页
Back interface passivation reduces the back recombination of photogenerated electrons, whereas aggravates the blocking of hole transport towards back contact, which complicate the back interface engineering for ultrat... Back interface passivation reduces the back recombination of photogenerated electrons, whereas aggravates the blocking of hole transport towards back contact, which complicate the back interface engineering for ultrathin CIGSe solar cells with a Schottky back contact. In this work, theoretical explorations were conducted to study how the two contradictory electrical effects impact cell performance. For ultrathin CIGSe solar cells with a pronounced Schottky potential barrier(E_(h)> 0.2 eV), back interface passivation produces diverse performance evolution trends, which are highly dependent on cell structures and properties. Since a back Ga grading can screen the effect of reduced recombination of photogenerated electrons from back interface passivation, the hole blocking effect predominates and back interface passivation is not desirable. However, when the back Schottky diode merges with the main pn junction due to a reduced absorber thickness,the back potential barrier and the hole blocking effect is much reduced on this occasion. Consequently, cells exhibit the same efficiency evolution trend as ones with an Ohmic contact, where back interface passivation is always advantageous.The discoveries imply the complexity of back interface passivation and provide guidance to manipulate back interface for ultrathin CIGSe solar on TCOs with a pronounced Schottky back contact. 展开更多
关键词 ultrathin cigse solar cells Schottky back contact back interface passivation back recombination hole blocking
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Non-exposed endoscopic wall-inversion surgery with one-step nucleic acid amplification for early gastrointestinal tumors:Personal experience and literature review
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作者 Francesco Crafa Serafino Vanella +7 位作者 Aristide Morante Onofrio A Catalano Kelsey L Pomykala Mario Baiamonte Maria Godas Alexandra Antunes Joaquim Costa Pereira Valentina Giaccaglia 《World Journal of Gastroenterology》 SCIE CAS 2023年第24期3883-3898,共16页
BACKGROUND Laparoscopic and endoscopic cooperative surgery is a safe,organ-sparing surgery that achieves full-thickness resection with adequate margins.Recent studies have demonstrated the safety and efficacy of these... BACKGROUND Laparoscopic and endoscopic cooperative surgery is a safe,organ-sparing surgery that achieves full-thickness resection with adequate margins.Recent studies have demonstrated the safety and efficacy of these procedures.However,these techniques are limited by the exposure of the tumor and mucosa to the peritoneal cavity,which could lead to viable cancer cell seeding and the spillage of gastric juice or enteric liquids into the peritoneal cavity.Non-exposed endoscopic wallinversion surgery(NEWS)is highly accurate in determining the resection margins to prevent intraperitoneal contamination because the tumor is inverted into the visceral lumen instead of the peritoneal cavity.Accurate intraoperative assessment of the nodal status could allow stratification of the extent of resection.One-step nucleic acid amplification(OSNA)can provide a rapid method of evaluating nodal tissue,whilst nearinfrared laparoscopy together with indocyanine green can identify relevant nodal tissue intraoperatively.AIM To determine the safety and feasibility of NEWS in early gastric and colon cancers and of adding rapid intraoperative lymph node(LN)assessment with OSNA.METHODS The patient-based experiential portion of our investigations was conducted at the General and Oncological Surgery Unit of the St.Giuseppe Moscati Hospital(Avellino,Italy).Patients with early-stage gastric or colon cancer(diagnosed via endoscopy,endoscopic ultrasound,and computed tomography)were included.All lesions were treated by NEWS procedure with intraoperative OSNA assay between January 2022 and October 2022.LNs were examined intraoperatively with OSNA and postoperatively with conventional histology.We analyzed patient demographics,lesion features,histopathological diagnoses,R0 resection(negative margins)status,adverse events,and follow-up results.Data were collected prospectively and analyzed retrospectively.RESULTS A total of 10 patients(5 males and 5 females)with an average age of 70.4±4.5 years(range:62-78 years)were enrolled in this study.Five patients were diagnosed with gastric cancer.The remaining 5 patients were diagnosed with early-stage colon cancer.The mean tumor diameter was 23.8±11.6 mm(range:15-36 mm).The NEWS procedure was successful in all cases.The mean procedure time was 111.5±10.7 min(range:80-145 min).The OSNA assay revealed no LN metastases in any patients.Histologically complete resection(R0)was achieved in 9 patients(90.0%).There was no recurrence during the follow-up period.CONCLUSION NEWS combined with sentinel LN biopsy and OSNA assay is an effective and safe technique for the removal of selected early gastric and colon cancers in which it is not possible to adopt conventional endoscopic resection techniques.This procedure allows clinicians to acquire additional information on the LN status intraoperatively. 展开更多
关键词 Laparoscopic and endoscopic cooperative surgery Non-exposed endoscopic wall inversion surgery Early gastric cancer Early colorectal cancer Sentinel lymph node One-step nucleic acid amplification Endoscopic full-thickness resection
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一种基于LMI的H_-/H_∞故障检测观测器优化设计方法 被引量:9
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作者 彭涛 桂卫华 +2 位作者 STEVEN X D 吴敏 李昊 《中南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2004年第4期628-631,共4页
针对线性时不变动态系统,构造一个线性全阶输出观测器,获得包括扰动、噪声、建模误差等未知输入信号和故障信号的残差动态特性,选用残差对故障信号的H-指数描述其对故障的灵敏度,用残差对未知信号的H∞范数表示其对不确定因素的鲁棒性,... 针对线性时不变动态系统,构造一个线性全阶输出观测器,获得包括扰动、噪声、建模误差等未知输入信号和故障信号的残差动态特性,选用残差对故障信号的H-指数描述其对故障的灵敏度,用残差对未知信号的H∞范数表示其对不确定因素的鲁棒性,将故障检测观测器的设计描述为一个优化问题。研究了基于线性矩阵不等式的故障检测观测器设计,给出了求解最优观测器增益的方法,并证明了该解存在的条件。实践结果表明,应用该方法所设计的故障检测观测器灵敏度高,对干扰信号具有强鲁棒性。 展开更多
关键词 故障检测 观测器 鲁棒性 灵敏度 线性矩阵不等式
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基于EEMD和SVM的冷轧机垂直振动相关故障的诊断 被引量:7
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作者 杨旭 彭开香 +3 位作者 罗浩 KRUEGER Min-jia 宗大桥 DING Steven X 《上海交通大学学报》 EI CAS CSCD 北大核心 2015年第6期751-756,共6页
通过对冷板带轧机垂直振动过程的机理进行分析,结合轧机系统结构模型,建立含振动因素的冷轧机垂向系统动态轧制力模型.考虑复杂工况下,轧机在生产不同规格带钢时,由工艺参数波动等广义故障所致轧机垂直振动现象,基于工业现场数据进行数... 通过对冷板带轧机垂直振动过程的机理进行分析,结合轧机系统结构模型,建立含振动因素的冷轧机垂向系统动态轧制力模型.考虑复杂工况下,轧机在生产不同规格带钢时,由工艺参数波动等广义故障所致轧机垂直振动现象,基于工业现场数据进行数据驱动的故障诊断算法研究.采用集成经验模态分解算法对实测轧制力信号进行分解,选取有效的固有模态函数的能量作为特征向量,并将其输入到支持向量机分类器中,通过分类器对正常状态和故障状态进行区分,以实现轧机振动相关故障的准确诊断. 展开更多
关键词 轧机 垂直振动 故障诊断 支持向量机 信号处理
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核废料储罐近域环境中温湿度长期演变预测 被引量:4
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作者 黄彦良 郑珉 +4 位作者 张琦超 路东柱 王秀通 KUNTE Hans-Jorg SAND Wolfgang 《装备环境工程》 CAS 2018年第10期109-113,共5页
为获得核废料储罐与周围环境界面的温湿度信息,以便模拟不同地质处置时期储罐表面的腐蚀环境,通过对一些典型核能利用国家在高放核废料安全处置研究中对储罐表面温度的长期演变计算进行调研,结合不同埋藏模式推测出适于我国的核废料储... 为获得核废料储罐与周围环境界面的温湿度信息,以便模拟不同地质处置时期储罐表面的腐蚀环境,通过对一些典型核能利用国家在高放核废料安全处置研究中对储罐表面温度的长期演变计算进行调研,结合不同埋藏模式推测出适于我国的核废料储罐表面温度演变规律。同时根据国内外对核废料储库中缓冲/回填材料饱和度的变化情况推测我国核废料储罐表面环境中膨润土含水量的时间演变趋势。研究表明,储罐表面初始时温度快速升高,至温度峰值后逐渐下降,为安全起见,最高温应设计低于100℃。膨润土的饱和度受到核废料衰变释热及地下水入渗的双重影响,早期以核废料衰变主导,后来受地下水入渗影响显著。一般认为储罐表面膨润土在3年左右含水量明显增加,约10年左右达到饱和。温湿度长期演变预测将为我国核废料储罐表面腐蚀演变研究奠定基础。 展开更多
关键词 核废料 处置库 温湿度 腐蚀演变
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核废料储罐表面化学环境长期演变预测
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作者 黄彦良 郑珉 +4 位作者 张琦超 路东柱 王秀通 KUNTE Hans-Jorg SAND Wolfgang 《装备环境工程》 CAS 2018年第10期103-108,共6页
通过对国内外的核废料处置库环境试验监测及预测模型的调研,总结了核废料储罐在直接填埋、缓冲/回填和加混凝土缓冲层等埋藏方式下的表面环境变化,主要包括氧含量、缓冲材料的孔隙液成分及pH值,为我国核废料储库环境下储罐表面重要的腐... 通过对国内外的核废料处置库环境试验监测及预测模型的调研,总结了核废料储罐在直接填埋、缓冲/回填和加混凝土缓冲层等埋藏方式下的表面环境变化,主要包括氧含量、缓冲材料的孔隙液成分及pH值,为我国核废料储库环境下储罐表面重要的腐蚀演变研究提供了参考。 展开更多
关键词 核废料 处置库 腐蚀环境 腐蚀演变
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四效逆流降膜蒸发系统的建模与故障仿真
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作者 胡志坤 李哲彬 +2 位作者 陈志文 桂卫华 阳春华 《中南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2015年第12期4504-4511,共8页
针对氧化铝生产蒸发过程难以控制、故障数据难以采集的现象,根据热力学的基本原理,利用奥秘仿真系统,用高保真的模型元件通过模块化的方法建立四效逆流降膜蒸发加三级闪蒸的氧化铝生产蒸发过程模型。根据氧化铝蒸发系统的特点,将模型分... 针对氧化铝生产蒸发过程难以控制、故障数据难以采集的现象,根据热力学的基本原理,利用奥秘仿真系统,用高保真的模型元件通过模块化的方法建立四效逆流降膜蒸发加三级闪蒸的氧化铝生产蒸发过程模型。根据氧化铝蒸发系统的特点,将模型分为4个蒸发器模块、3个预热器模块和3个闪蒸器模块。为了体现系统的中间过程,模型中把蒸发器模块分为蒸汽单元和物料单元。通过软件中模型计算和仿真验证模型的有效性,与氧化铝蒸发过程实际测量值相比较,证明模型能够很好地反映氧化铝蒸发的动态生产过程。此外,模型还设置一些实际过程中常发生的故障。研究结果表明:通过数据分析发现故障仿真结果与理论结果相符合,为故障检测和优化控制系统奠定了基础。 展开更多
关键词 蒸发过程 模块化 故障仿真
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AKT and ERK1/2 signaling in intrahepatic cholangiocarcinoma 被引量:9
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作者 KJ Schmitz H Lang +4 位作者 J Wohlschlaeger GC Sotiropoulos H Reis KW Schmid HA Baba 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第48期6470-6477,共8页
Intrahepatic cholangiocarcinomas (ICC) are neoplasms that originate from cholangiocytes and can occur at any level of the biliary tree. Surgical resection is the current therapy of choice for this highly aggressive ca... Intrahepatic cholangiocarcinomas (ICC) are neoplasms that originate from cholangiocytes and can occur at any level of the biliary tree. Surgical resection is the current therapy of choice for this highly aggressive cancer. However, the 5-year survival still is poor, with high recurrence rates. Due to the intrahepatic growth a signifi cant proportion of patients present with advanced disease and are not candidates for curative surgery or transplantation. The existing palliative options are of limited benefit and there is a great necessity for novel therapeutic options. In this article we review the role of the phosphoinositide 3-kinase(PI3K)/ AKT and extracellular regulated kinase (ERK) signaling pathways in ICC and present new data on the prognostic value of these protein kinases. Finally, we discuss future upcoming therapeutic options based on targeting these signaling pathways. 展开更多
关键词 Extra cellular regulated kinases CHOLANGIOCARCINOMA Prognosis ONCOLOGY IMMUNOHISTOCHEMISTRY
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Placebo responses in patients with gastrointestinal disorders 被引量:7
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作者 Frauke Musial Sibylle Klosterhalfen Paul Enck 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第25期3425-3429,共5页
Over the last several years there has been a growing interest in placebo, not only as an inert control in clinical trials, but also in the placebo effect as a group effect as well as a reaction in individual subjects.... Over the last several years there has been a growing interest in placebo, not only as an inert control in clinical trials, but also in the placebo effect as a group effect as well as a reaction in individual subjects. Methodological factors such as regression to the mean and natural history of the disease play a role in the evaluation of a possible placebo effect. In this report, we discuss several factors including PavIovian conditioning, beliefs outcome, expectations, and other factors as potential mediators of the placebo response. Placebo effects are common in gastrointestinal diseases and there seems to be no clear difference between placebo effects in functional gastrointestinal diseases (functional dyspepsia and irritable bowel syndrome) and organic gastrointestinal disease (duodenal ulcer and inflammatory bowel disease). 展开更多
关键词 PLACEBO Gastrointestinal disease Regressionto the mean Natural history PavIovian conditioning Outcome expectation BELIEFS
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New therapeutic vaccination strategies for the treatment of chronic hepatitis B 被引量:9
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作者 Jia Liu Anna Kosinska +1 位作者 Mengji Lu Michael Roggendorf 《Virologica Sinica》 SCIE CAS CSCD 2014年第1期10-16,共7页
Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in o... Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in only about 30%patients and is associated with side effects.Most patients receiving nucleot(s)ide analogue treatment do not establish long-term,durable control of Infection and have rebounding viremia after cessation of therapy.Thus,novel therapy strategies are necessary to achieve the induction of potent and durable antiviral immune responses of the patients which can maintain long-term control of viral replication.Therapeutic vaccination of HBV carriers is a promising strategy for the control of hepatitis B.Here the authors review new therapeutic vaccination strategies to treat chronic hepatitis B which may be introduced for patient treatment in the future. 展开更多
关键词 hepatitis B virus woodchuck hepatitis virus therapeutic vaccination IMMUNOMODULATION programmed death-1
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Utilization of fly ash from coal-fired power plants in China 被引量:12
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作者 Eva SELIC Jan-Dirk HERBELL 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2008年第5期681-687,共7页
The rapidly increasing demand for energy in China leads to the construction of new power plants all over the country. Coal, as the main fuel resource of those power plants, results in increasing problems with the disp... The rapidly increasing demand for energy in China leads to the construction of new power plants all over the country. Coal, as the main fuel resource of those power plants, results in increasing problems with the disposal of solid residues from combustion and off gas cleaning. This investigation describes chances for the utilization of fly ash from coal-fired power plants in China. After briefly comparing the situation in China and Germany, the status of aluminum recycling from fly ash and the ad- vantages for using fly ash in concrete products are introduced. Chemical and physical analyses of Chinese fly ash samples, e.g., X-ray diffraction (XRD), ICP (Inductive Coupled Plasma) and particle size analysis, water requirement, etc. are presented. Rea- sonable amounts of aluminum were detected in the samples under investigation, but for recovery only sophisticated procedures are available up to now. Therefore, simpler techniques are suggested for the first steps in the utilization of Chinese fly ash. 展开更多
关键词 Fly ash utilization Aluminum recycling CONCRETE Chemical and physical analyses
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Bcl-2 degradation is an additional pro-apoptotic effect of polo-like kinase inhibition in cholangiocarcinoma cells 被引量:5
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作者 Svenja Sydor Sami Jafoui +6 位作者 Lena Wingerter Sandra Swoboda Joachim C Mertens Guido Gerken Ali Canbay Andreas Paul Christian D Fingas 《World Journal of Gastroenterology》 SCIE CAS 2017年第22期4007-4015,共9页
To examine the influence on apoptotic mechanisms following inhibition of polo-like kinases as therapeutically approach for cholangiocellular cancer treatment.METHODSAs most cholangiocarcinomas are chemotherapy-resista... To examine the influence on apoptotic mechanisms following inhibition of polo-like kinases as therapeutically approach for cholangiocellular cancer treatment.METHODSAs most cholangiocarcinomas are chemotherapy-resistant due to mechanisms preventing tumor cell death, we investigated the effect of Cisplatin on cholangiocellular carcinoma (CCA) cell lines KMCH-1 and Mz-Ch-1. Polo-like kinases (PLK) are important regulators of the cell cycle and their inhibition is discussed as a potential therapy while PLK inhibition can regulate apoptotic mediators. Here, cells were treated with PLK inhibitor BI6727 (Volasertib), Cisplatin, and in combination of both compounds. Cell viability was assessed by MTT; apoptosis was measured by DAPI staining and caspase-3/-7 assay. Western blot and qRT-PCR were used to measure expression levels of apoptosis-related molecules Bax and Bcl-2.RESULTSThe cell viability in the CCA cell lines KMCH-1 and Mz-Ch-1 was reduced in all treatment conditions compared to vehicle-treated cells. Co-treatment with BI6727 and cisplatin could even enhance the cytotoxic effect of cisplatin single treatment. Thus, co-treatment of cisplatin with BI6727 could slightly enhance the cytotoxic effect of the cisplatin in both cell lines whereas there was evidence of increased apoptosis induction solely in Mz-Ch-1 as compared to KMCH-1. Moreover, PLK inhibition decreases protein levels of Bcl-2; an effect that can be reversed by the proteasomal degradation inhibitor MG-132. In contrast, protein levels of Bax were not found to be altered by PLK inhibition. These findings indicate that cytotoxic effects of Cisplatin in Mz-Ch-1 cells can be enhanced by cotreatment with BI6727.CONCLUSIONIn conclusion, BI6727 treatment can sensitize CCA cells to cisplatin-induced apoptosis with proteasomal Bcl-2 degradation as an additional pro-apoptotic effect. 展开更多
关键词 Tumor necrosis factor-related apoptosis-inducing ligand Myeloid cell leukemia-1 Hedgehog pathway CISPLATIN Chemotherapy resistance
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Oxidative stress: the lowest common denominator of multiple diseases 被引量:7
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作者 Veronika Matschke Carsten Theiss Johann Matschke 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第2期238-241,共4页
Oxygen is essential to the human life and life of all aerobic organisms. The complete oxidation of nutrients for the biological energy supply is one of the most important prerequisites for the formation of higher life... Oxygen is essential to the human life and life of all aerobic organisms. The complete oxidation of nutrients for the biological energy supply is one of the most important prerequisites for the formation of higher life forms. However, cells that benefit from oxidative respiration also suffer from reactive oxygen species because they adapted to oxygen as an energy source. Healthy cells balance the formation and elimination of reactive oxygen species thereby creating and keeping reactive oxygen species-homeostasis. When the concentration of free radicals exceeds a critical level and homeostasis is disturbed, oxidative stress occurs leading to damage of multiple cellular molecules and compartments. Therefore, oxidative stress plays an important role in the physiology and pathology of various diseases. Often, the antioxidant protection system becomes pathologically unbalanced in the genesis of several diseases, leading to functional losses of the organism, as in the case of amyotrophic lateral sclerosis, or cells develop metabolic mechanisms to use this system as protection against external influences, such as in the case of glioblastoma cells. Either way, understanding the underlying deregulated mechanisms of the oxidative protection system would allow the development of novel treatment strategies for various diseases. Thus, regardless of the direction in which the reactive oxygen species-homeostasis disequilibrate, the focus should be on the oxidative protection system. 展开更多
关键词 NEURODEGENERATIVE disease amyotrophic lateral SCLEROSIS cancer GLIOBLASTOMA reactive oxygen species METABOLISM ANTIOXIDANT protection system
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P-glycoprotein multidrug transporter in inflammatory bowel diseases: More questions than answers 被引量:7
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作者 Elke Cario 《World Journal of Gastroenterology》 SCIE CAS 2017年第9期1513-1520,共8页
The gastrointestinal barrier is constantly exposed to numerous environmental substrates that are foreign and potentially harmful. These xenobiotics can cause shifts in the intestinal microbiota composition, affect muc... The gastrointestinal barrier is constantly exposed to numerous environmental substrates that are foreign and potentially harmful. These xenobiotics can cause shifts in the intestinal microbiota composition, affect mucosal immune responses, disturb tissue integrity and impair regeneration. The multidrug transporter ABCB1/MDR1 p-glycoprotein (p-gp) plays a key role at the front line of host defence by efficiently protecting the gastrointestinal barrier from xenobiotic accumulation. This Editorial discusses how altered expression and function of ABCB1/MDR1 p-gp may contribute to the development and persistence of chronic intestinal inflammation in inflammatory bowel diseases (IBD). Recent evidence implies multiple interactions between intestinal microbiota, innate immunity and xenobiotic metabolism via p-gp. While decreased efflux activity may promote disease susceptibility and drug toxicity, increased efflux activity may confer resistance to therapeutic drugs in IBD. Mice deficient in MDR1 A develop spontaneously chronic colitis, providing a highly valuable murine IBD model for the study of intestinal epithelial barrier function, immunoregulation, infectious co-triggers and novel therapeutic approaches. Possible associations of human ABCB1 gene polymorphisms with IBD susceptibility have been evaluated, but results are inconsistent. Future studies must focus on further elucidation of the pathophysiological relevance and immunological functions of p-gp and how its ambiguous effects could be therapeutically targeted in IBD. 展开更多
关键词 Inflammatory bowel diseases Multidrug resistance Innate immunity MICROBIOTA Xenobiotics
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Fault Detection of Networked Control Systems Based on Optimal Robust Fault Detection Filter 被引量:11
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作者 WANG Yong-Qiang YE Hao +1 位作者 DING X. Steven WANG Gui-Zeng 《自动化学报》 EI CSCD 北大核心 2008年第12期1534-1539,共6页
有可能比一个采样时期大的随机、未知的导致网络的延期的联网的控制系统(NCS ) 的差错察觉在这份报纸被学习。首先,导致网络的延期引起的影响被转变成为错误建模组织,然后存在连续时间域基于引用,模型被扩大到分离时间领域并且适用... 有可能比一个采样时期大的随机、未知的导致网络的延期的联网的控制系统(NCS ) 的差错察觉在这份报纸被学习。首先,导致网络的延期引起的影响被转变成为错误建模组织,然后存在连续时间域基于引用,模型被扩大到分离时间领域并且适用指责联网的控制系统的察觉的柔韧的差错察觉方法。建议方法能被 Matlab LMI 工具箱容易实现,并且它的表演被一个模拟例子最后评估。 展开更多
关键词 检测方法 网络控制系统 最优鲁棒故障检测系统 滤波器
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