Analysis of images obtained from two-dimensional gel electrophoresis （2D-GE） is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be ne...Analysis of images obtained from two-dimensional gel electrophoresis （2D-GE） is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be neither completely effective nor fully automatic, often requiring manual revision and refinement of computer generated matches. In this work, we present an effective technique for the detection and the reconstruction of over-saturated protein spots. Firstly, the algorithm reveals overexposed areas, where spots may be truncated, and plateau regions caused by smeared and overlapping spots. Next, it reconstructs the correct distribution of pixel values in these overexposed areas and plateau regions, using a two-dimensional least-squares fitting based on a generalized Gaussian distribution. Pixel correction in saturated and smeared spots allows more accurate quantification, providing more reliable image analysis results. The method is validated for processing.highly exposed 2D-GE images, comparing reconstructed spots with the corresponding non-saturated image, demonstrating that the algorithm enables correct spot quantification.展开更多
The notion of Lp-dichotomy for linear differential equations with possibly unbounded oper-ator is introduced. By help of Banach fixed point theorem sufficient conditions for the existenceof bounded solutions of nonlin...The notion of Lp-dichotomy for linear differential equations with possibly unbounded oper-ator is introduced. By help of Banach fixed point theorem sufficient conditions for the existenceof bounded solutions of nonlinear differential equations with an Lp-dichotomous linear part areobtained.展开更多
During ladle stirring,a gas is injected into the steel bath to generate a mixing of the liquid steel.The optimal process control requires a reliable measurement of the stirring intensity,for which the induced ladle wa...During ladle stirring,a gas is injected into the steel bath to generate a mixing of the liquid steel.The optimal process control requires a reliable measurement of the stirring intensity,for which the induced ladle wall vibrations have proved to be a potential indicator.An experimental cold water ladle with two eccentric nozzles and eight mono-axial accelerometers was thus investigated to measure the vibrations.The effect of the sensors'positions with respect to the gas plugs on the vibration intensity was analyzed,and experimental data on several points of the ladle were collected for future numerical simulations.It is shown that the vibration root-mean-square values depend not only on process parameters,such as gas flow rate,water,and oil heights,but also on the radial and axial positions of the sensors.The vibration intensity is clearly higher,close to the gas plumes,than in the opposite side.If one of the nozzles is clogged,the vibration intensity close to the clogged nozzle drops drastically(-36 to-59%),while the vibrations close to the normal operating nozzle are hardly affected.Based on these results,guidelines are provided for an optimized vibration-based stirring.展开更多
基金funded by the Valle d’Aosta Regional Government (http://www.regione.vda.it/) in the frame of the regional law n.84-07/12/1993 (project ParIS-Parkinson Informative System)
文摘Analysis of images obtained from two-dimensional gel electrophoresis （2D-GE） is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be neither completely effective nor fully automatic, often requiring manual revision and refinement of computer generated matches. In this work, we present an effective technique for the detection and the reconstruction of over-saturated protein spots. Firstly, the algorithm reveals overexposed areas, where spots may be truncated, and plateau regions caused by smeared and overlapping spots. Next, it reconstructs the correct distribution of pixel values in these overexposed areas and plateau regions, using a two-dimensional least-squares fitting based on a generalized Gaussian distribution. Pixel correction in saturated and smeared spots allows more accurate quantification, providing more reliable image analysis results. The method is validated for processing.highly exposed 2D-GE images, comparing reconstructed spots with the corresponding non-saturated image, demonstrating that the algorithm enables correct spot quantification.
文摘The notion of Lp-dichotomy for linear differential equations with possibly unbounded oper-ator is introduced. By help of Banach fixed point theorem sufficient conditions for the existenceof bounded solutions of nonlinear differential equations with an Lp-dichotomous linear part areobtained.
文摘During ladle stirring,a gas is injected into the steel bath to generate a mixing of the liquid steel.The optimal process control requires a reliable measurement of the stirring intensity,for which the induced ladle wall vibrations have proved to be a potential indicator.An experimental cold water ladle with two eccentric nozzles and eight mono-axial accelerometers was thus investigated to measure the vibrations.The effect of the sensors'positions with respect to the gas plugs on the vibration intensity was analyzed,and experimental data on several points of the ladle were collected for future numerical simulations.It is shown that the vibration root-mean-square values depend not only on process parameters,such as gas flow rate,water,and oil heights,but also on the radial and axial positions of the sensors.The vibration intensity is clearly higher,close to the gas plumes,than in the opposite side.If one of the nozzles is clogged,the vibration intensity close to the clogged nozzle drops drastically(-36 to-59%),while the vibrations close to the normal operating nozzle are hardly affected.Based on these results,guidelines are provided for an optimized vibration-based stirring.
