Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laborat...Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.展开更多
西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中...西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中脊热液区的硫化物和围岩等样品进行了系统的物性测量,结合岩石物性(包括密度、孔隙度、P波速度)与矿物组成,深入分析了西南印度洋中脊热液区岩石声速变化特性及其影响因素。结果表明,SWIR热液区围岩的P波速度受到岩石骨架矿物、孔隙和围压的影响。由于岩石孔隙度总体偏小,对P波速度的影响并不显著,但围压的增加使岩石微裂缝和孔隙逐渐闭合,P波速度呈非线性指数变化。蚀变作用导致了矿物成分改变,是影响围岩声速的最关键因素。单一物性参数测量结果可能存在多解性,联合波速、密度、磁性和电性等多物性参数测量有利于岩性区分。该研究成果有助于识别硫化物和围岩,为我国西南印度洋合同区多金属硫化物地震勘探工作提供重要支撑。展开更多
Utilization of urban underground space has become a vital approach to alleviate the strain on urban land resources,and to optimize the structure and pattem of the city.It is also very important to improve the city env...Utilization of urban underground space has become a vital approach to alleviate the strain on urban land resources,and to optimize the structure and pattem of the city.It is also very important to improve the city environment,build livable city and increase the capacity of the city.Based on the analysis of existing evaluation methods and their problems,a method for evaluating underground space resources based on a negative list of adverse factors affecting underground space development is proposed,to be primarily used in urban planning stages.A list of the adverse factors is established,including limiting factors,constraining factors and influencing factors.Taking Xi'an as an example,using a geographical information system platform,a negative list of adverse factors for the underground space resources in Xi'an City are evaluated,and preventive measures are proposed.Natural resources,exploitable resources,and the potential growth of exploitable underground space resources are evaluated.Underground space assessment in the different development stages of the city,collaborative utilization and safety evaluation for multiple subsurface resources,environmental impact and assessment,as well as evaluation methods based on big data and intelligent optimization algorithms are all discussed with the aim of serving city planning and construction.展开更多
There are lots of theories about the causation of ground fissures in Xi'an,such as the tectonics theory,the excessive groundwater exploitation theory and the compositive theory. Based on the construction of the ge...There are lots of theories about the causation of ground fissures in Xi'an,such as the tectonics theory,the excessive groundwater exploitation theory and the compositive theory. Based on the construction of the geologic environment monitoring network in Guanzhong urban agglomeration,the latest survey of ground fissures and land subsidence in Yuhuazhai in Xi'an shows that the extensive piping and quicksand in self-supply wells is a factor to induce ground fissures and land subsidence. It is suggested that the seepage deformation caused by high hydraulic gradient leads to sand gushing and changes some aquifers into the composite aquifer,which is the main factor to induce ground fissures and land subsidence. The development characteristics of ground fissures and land subsidence caused by seepage deformation were summarized. The results will supply new schemes and methods for the causation of land subsidence and ground fissures in Xi'an and lay out a clear road map of measures to control land subsidence and ground fissures.展开更多
基金supported by the National Key Research and Development Program(grant number:2022YFC2305304).
文摘Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.
文摘西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中脊热液区的硫化物和围岩等样品进行了系统的物性测量,结合岩石物性(包括密度、孔隙度、P波速度)与矿物组成,深入分析了西南印度洋中脊热液区岩石声速变化特性及其影响因素。结果表明,SWIR热液区围岩的P波速度受到岩石骨架矿物、孔隙和围压的影响。由于岩石孔隙度总体偏小,对P波速度的影响并不显著,但围压的增加使岩石微裂缝和孔隙逐渐闭合,P波速度呈非线性指数变化。蚀变作用导致了矿物成分改变,是影响围岩声速的最关键因素。单一物性参数测量结果可能存在多解性,联合波速、密度、磁性和电性等多物性参数测量有利于岩性区分。该研究成果有助于识别硫化物和围岩,为我国西南印度洋合同区多金属硫化物地震勘探工作提供重要支撑。
基金This research is supported by National Key Research and Development Project,Ministry of Science and Technology of the People’s Republic of China(2018YFC1504700).
文摘Utilization of urban underground space has become a vital approach to alleviate the strain on urban land resources,and to optimize the structure and pattem of the city.It is also very important to improve the city environment,build livable city and increase the capacity of the city.Based on the analysis of existing evaluation methods and their problems,a method for evaluating underground space resources based on a negative list of adverse factors affecting underground space development is proposed,to be primarily used in urban planning stages.A list of the adverse factors is established,including limiting factors,constraining factors and influencing factors.Taking Xi'an as an example,using a geographical information system platform,a negative list of adverse factors for the underground space resources in Xi'an City are evaluated,and preventive measures are proposed.Natural resources,exploitable resources,and the potential growth of exploitable underground space resources are evaluated.Underground space assessment in the different development stages of the city,collaborative utilization and safety evaluation for multiple subsurface resources,environmental impact and assessment,as well as evaluation methods based on big data and intelligent optimization algorithms are all discussed with the aim of serving city planning and construction.
基金Supported by Comprehensive Geological Survey of Guanzhong-Tianshui Economic Zone(Zhongdidiaoshen[2016]05027)Construction of Geological Environment Monitoring Network in the Guanzhong Basin(Shui[2016](7))
文摘There are lots of theories about the causation of ground fissures in Xi'an,such as the tectonics theory,the excessive groundwater exploitation theory and the compositive theory. Based on the construction of the geologic environment monitoring network in Guanzhong urban agglomeration,the latest survey of ground fissures and land subsidence in Yuhuazhai in Xi'an shows that the extensive piping and quicksand in self-supply wells is a factor to induce ground fissures and land subsidence. It is suggested that the seepage deformation caused by high hydraulic gradient leads to sand gushing and changes some aquifers into the composite aquifer,which is the main factor to induce ground fissures and land subsidence. The development characteristics of ground fissures and land subsidence caused by seepage deformation were summarized. The results will supply new schemes and methods for the causation of land subsidence and ground fissures in Xi'an and lay out a clear road map of measures to control land subsidence and ground fissures.
